褪黑激素对内蒙古绒山羊初级毛囊体外培养影响的研究
Effect of melatonin on Inner Mongolia primary follicle growth and development

为探究褪黑激素（MT）对绒山羊毛囊体外生长及毛囊形态的影响，采用物理切割法结合胶原酶消化分离绒山羊初级毛囊，并通过设置酶的不同体积分数（0.10%、0.20%、0.25%、0.30%、0.35%、0.40%和0.45%）以及相应的消化时间（100、80、70、60、50、45和35 min）确定型胶原酶消化的最佳条件。在初级毛囊体外培养的基础上，添加褪黑激素（300 pg/mL）连续培养5 d，在显微镜下观察毛囊每天的生长情况。结果显示，毛囊分离过程中胶原酶消化的最佳体积分数和时间为0.30%、60 min，褪黑激素对初级毛囊体外生长具有极显著的（P<0.01）促进作用。
To study the effects of melatonin (MT) on the fiber growth and follicle morphology of primary follicle from Inner Mongolia cashmere goat in vitro,microdissection separation method and enzyme digestion method was used to isolate primary follicles.The primary follicle was isolated in different concentrations of collagenase (0.10%,0.20%,0.25%,0.30%,0.35%,0.40% and 0.45%) and at different time (100,80,70,60,50,45 and 35 min).Melatonin (300 pg/mL) was added to primary follicle culture for 5 days,and then primary follicle was observed with an inverted phase-contrast microscope.The results showed that the most suitable concentration and time was 0.3% and 60 min,and the effect of melatonin on primary hair follicles was significant (P<0.01).