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- 2018
纳米Fe3O4-还原氧化石墨烯复合修饰玻碳电极的制备及电化学检测多巴胺
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Abstract:
通过电化学还原法制备纳米Fe3O4-还原氧化石墨烯复合修饰玻碳（Fe3O4-rGO/GCE）电极，用于多巴胺（DA）的检测。采用SEM、TEM和循环伏安对纳米Fe3O4-rGO复合材料进行表征。在pH为7.0的磷酸盐缓冲液（PBS）中，采用循环伏安法研究了DA在纳米Fe3O4-rGO/GC上的电化学行为。实验结果表明，较裸GC电极和rGO修饰（rGO/GC）电极，由于纳米Fe3O4与rGO的协同作用，纳米Fe3O4-rGO/GC显著增大了Fe3O4-rGO/GC复合材料电极电化学活性面积和氧化峰电流强度ipa。DA的浓度在6.0×10-8~2.0×10-6 mol/L和2.0×10-6~8.0×10-5 mol/L范围内，与氧化峰电流强度ipa呈良好的线性关系，检出限达4.0×10-9 mol/L（信噪比S/N=3）。抗坏血酸和尿酸共存物几乎不干扰DA的测定，选择性高。Fe3O4-rGO/GC修饰电极用于盐酸DA注射液中的DA含量测定，获得结果较好，回收率为97.1%~103.9%。 The nano Fe3O4-reduced graphene oxide composite modified glassy carbon (Fe3O4-rGO/GC) electrode was fabricated by electrochemical reduction method, and used for the determination of dopamine (DA).The nano Fe3O4-rGO composites were characterized by SEM, TEM and cyclic voltammetry. The electrochemical behavior of DA was investigated in pH=7.0 phosphate buffer saline (PBS) by cyclic voltammetry. Compared with bare GC electrode and rGO-modified (rGO/GC) electrode, the electrochemical active area and the oxide peak current ipa of Fe3O4-rGO/GC electrode increase significantly, owing to the synergistic action between nano Fe3O4 and rGO. The oxide peak current ipa shows good linear relationship with the concentration of DA in the range of 6×10-8-2×10-6 mol/L and 2×10-6-8×10-5 mol/L. The detection limit is 4.0×10-9 mol/L (signal-noise ratio S/N=3).The determination of DA hardly interfere with the coexistence of ascorbic acid and uric acid. The proposed method is further applied to the determination of DA in DA injections with satisfied results, and the recovery of DA is 97.1%-103.9%. 国家自然科学基金（61703152）；湖南工业大学博士学科建设项目；湖南省自然科学基金（2016JJ4010）；株洲科技计划项目；衡阳师范学院省级平台开放基金（GD16K02）
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