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- 2017
负载BMP-2和BSA的丝素蛋白/聚左旋乳酸-己内酯三维纤维屏障膜的制备及性能
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Abstract:
利用骨形态发生蛋白-2（BMP-2）的骨诱导作用和牛血清白蛋白（BSA）的稳定作用，结合同轴静电纺丝技术和共混静电纺丝技术，制备了三组纤维膜，包括同时携载BMP-2和BSA的丝素蛋白（SF）/聚左旋乳酸-己内酯（PLCL）同轴载药纤维膜（BMP-2-BSA@SF/PLCL，Mat A）；同时携载BMP-2和BSA的SF/PLCL共混载药纤维膜（BMP-2-BSA-SF/PLCL，Mat B）和非载药SF/PLCL纤维膜（SF/PLCL，Mat C）。通过研究纤维膜的表观形态，得出三种纤维膜均呈均匀网状结构，其中Mat A组具有稳定的核层结构；理化性能研究证实三组纤维的接触角和力学性能依次增大；体外细胞实验和膜屏障实验表明，三组纤维膜均具有良好的生物相容性和屏障功能，且具有药物缓释作用的Mat A组纤维膜能够更加有效地刺激细胞生长和早期骨向分化。所制备的BMP-2-BSA@SF/PLCL能够满足引导性骨组织再生（GBR）生物膜的基本要求，是促进骨缺损修复的理想载体。 Due to theosteoinduction of the bone morphogenetic protein-2 (BMP-2) and stabilization of bovine serum albumin (BSA), three types of new biological mat was fabricated, namely silk fibroin (SF)/poly (L-lactide-co-caprolactone)(PLCL) mat loaded with BMP-2 and BSA by coaxial electrospinning (BMP-2-BSA@SF/PLCL, Mat A), SF/PLCL mat loaded with BMP-2 and BSA by blend electrospinning (BMP-2-BSA-SF/PLCL, Mat B), non-loaded SF/PLCL mat (SF/PLCL, Mat C). The results of apparent characteristics show all the fibrous mats present homogeneous network structure, and the core-shell structure is formed only in Mat A. The physicochemical properties results show contact angle and mechanical properties of Mat A, Mat B and Mat C increase in turn. Although each group has good biocompatibility and barrier function, Mat A which has the ability of sustained drugrelease can stimulate growth and osteogenic differentiation of cells more effectively. The BMP-2-BSA@SF/PLCL fibrous mat can meet the requirements of guided bone regeneration (GBR) and will be used as the ideal carrier for bone defect repair. 教育部"中央高校基本科研业务费专项资金"重点项目（lzujbky-2016-k17）；兰州市人才创新创业项目（2014-RC-79）
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