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- 2018
氧化石墨烯/纳米羟基磷灰石复合粉体的制备及其细胞毒性
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Abstract:
采用改进Hummers法制备了氧化石墨烯（GO），再与Ca（NO3）2和（NH4）2HPO4通过溶液离子共滴定法制备GO/纳米羟基磷灰石（nHAP）复合粉体材料。利用TEM、XRD、FTIR、XPS等分析了GO/nHAP复合粉体的形貌、相结构、官能团以及元素化学价态变化，最后通过3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴（MTT）法对GO/nHAP粉体复合材料的生物学性能进行检测。结果表明：GO/nHAP复合粉体中nHAP呈现晶簇针状，且分散均匀，尺寸约为50~100 nm，GO片层尺寸为1 000~4 000 nm；nHAP在GO表层原位形核，并沿着（112）晶面择优生长，两相通过化学键的形式结合；GO/nHAP复合粉体材料无细胞毒性。 The graphene oxide (GO) was synthesized by the modified Hummers' method, then GO and Ca(NO3)2 and (NH4)2HPO4 were prepared to synthesize the GO/nano hydroxyapatite (nHAP) composites powder by simultaneous titration method. The morphology, phases, chemical groups and element valence of the GO/nHAP composites powder were investigated by TEM, XRD, FTIR and XPS, and the biological performance of GO/nHAP composite powder was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The results show that the nHAP in the GO/nHAP composites appears drusy acicular crystal structure and disperses evenly. The size of drusy acicular crystals is about 50-100 nm, and the size of graphene oxide sheets is 1 000-4 000 nm. nHAP nucleates in situ on the surface of the GO and grows along the (112) plane preferentially, and the two phases are bound to each other by chemical bonds. The GO/nHAP composites powder exhibit no cytotoxicity. 国家自然科学基金（31260228）
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