维生素D受体基因多态性与慢性牙周炎的相关性
Relationship between vitamin D receptor gene polymorphisms and chronic periodontitis

目的：探讨中国汉族维生素D受体（vitamin D receptor, VDR）基因多态性与慢性牙周炎的关系。方法：选取105例轻/中度慢性牙周炎和85例重度慢性牙周炎患者，采集口腔颊黏膜拭子，提取DNA，应用基质辅助激光解吸电离飞行时间质谱技术（matrix-assisted laser desorption ionization time-of-flight, MALDI-TOF）检测VDR基因的多态性。结果：在受检总人群及女性患者中，rs1544410的A等位基因及AA+AG基因型在重度牙周炎组检出率高于轻/中度组（总人群：P=0.006，0.007；女性患者：P=0.001，0.001）；rs731236的C等位基因及CC+CT基因型在重度牙周炎组检出率高于轻/中度组（总人群：P=0.003，0.004；女性患者：P<0.001，<0.001）。结论：VDR的rs731236和rs1544410基因多态性可能是中国汉族重度慢性牙周炎的危险因素，VDR多态性对牙周炎程度的影响可能存在性别差异。
Objective：To investigate the relationship between vitamin D receptor (VDR) gene polymorphisms and chronic periodontitis(CP). Methods: Buccal swabs from 105 patients with mild/moderate CP and 85 severe CP were collected, DNA was extracted from these buccal swabs using the TIANamp Swab DNA Kit ［TIANGEN Biotech (Beijing) CO.Ltd］. The VDR rs1544410 and rs731236 were genotyped by the Sequenom MassARRAY system (Shanghai Benegene Biotechnology Co. Ltd), which was based on MALDITOF (matrixassisted laser desorption ionization timeofflight) technology. The distribution of the genotypes and allele frequencies were analyzed. Results: The frequencies of the rs1544410 A allele and AA+AG genotype were significantly higher in severe CP than in mild/moderate CP of all the patients and the female patients respectively (all the patients: P=0.006, 0.007; the female patients: P=0.001, 0.001). The frequencies of the rs731236 C allele and CC+CT genotype were significantly higher in severe CP than in mild/moderate CP of all the patients and the female patients respectively (all the patients: P=0.003, 0.004; the female patients: P<0.001, <0.001). Conclusion: Gene polymorphisms of VDR rs731236 and rs1544410 may be associated with severe CP in Chinese Han population.