不同高度丝素蛋白支架体内原位修复兔膝关节骨软骨缺损的研究
Different Thickness Silk Fibroin Scaffolds Regenerating Osteochondral Defect in Situ

摘要：目的：通过研究不同高度丝素蛋白支架体内原位修复兔膝关节骨软骨缺损的修复机制，为临床应用提供研究基础。方法：将11只新西兰大白兔随机分为两组，包括高支架组（LONG，材料平关节面）、低支架组（SHORT，材料低于软骨下骨板）。在双侧膝关节股骨滑车处制造骨软骨缺损（深度3.5~4.0 mm，直径4.0mm），将2种支架分别植入双侧膝骨软骨缺损处。术后6周和12周时麻醉并处死动物，行大体形态观察、组织学观察及力学测试。结果：经过结构和功能的综合观察评估，低支架组中骨软骨缺损的修复效果优于高支架组。低支架所修复的关节面更平整，软骨下骨板生长更厚实且力学性能更优越。结论：低于软骨下骨板的丝素蛋白支架能较好地促进关节骨软骨缺损的修复，一定程度上提示着骨软骨支架新的应用方式。
Abstract: Objective: The purpose of this study was to investigate the effects of the different depth silk fibroin scaffolds on osteochondral regeneration and its possible mechanism for clinical?research. Methods: Eleven New Zealand rabbits were randomly divided into two groups, including long scaffold group (long, equal altitude with osteochondral defect) and short scaffold group (short, under the subcartilaginous osseous lamella). The osteochondral defects were made in bilateral femoral condyles of patellofemoral knees (3.5-4.0mm in thickness, 4.0 mm in diameter). Then long scaffold and short scaffold was transplanted into the defects respectively. Rabbits were anaesthetized and sacrificed at 6 weeks or 12 weeks after operation, morphology observation, histological observation and mechanical test were done. Results: The effect of short scaffold on osteochondral regeneration is better than that of long scaffold, cartilage surface regenerated by short scaffold grows more smoothly, its bone plate is thicker and has better mechanical property. Conclusion: Silk fibroin scaffold, shorter than subcartilaginous osseous lamella, can promote the regeneration of osteochondral defect, which implicates its potential new application