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- 2018
利用高效液相色谱法同时测定枇杷果实中10种植物激素
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Abstract:
以三倍体枇杷花和幼果为研究材料, 建立了一套可同时分离和测定玉米素、赤霉素、激动素、水杨酸、6-苄氨基腺嘌呤、苯甲酸、吲哚乙酸、脱落酸、吲哚丁酸和茉莉酸10种植物激素的方法.在甲醇:乙腈:磷酸缓冲液(pH值为3.0)体积比为20:20:60为流动相, 流速为1 mL/min, 柱温35 ℃, 波长为210 nm和254 nm条件下, 测定枇杷幼果中10种激素, 其线性相关系数在0.9915以上, 加标回收率在58.6%~85.2%之间; 检出限在0.004~4.92 ng之间, 精密度在0.026%~3.05%之间.此外, 利用该方法对番茄和甘蓝叶片中10种内源激素含量进行了验证, 表明该方法可用于植物中多种激素的同时测定.
In this study, a high-performance liquid chromatography method was established for simultaneously determining ten phytohormones (trans-zeatin, gibberellin A3, kinetin, salicylic acid, 6-benzylaminopurine, benzoic acid, indole-3-acetic acid, abscisic acid, indole-3-butyric acid and jasmonic acid) in triploid loquat (Eriobotrya japonica (Thunb) Lindl.) flowers and fruitlet.The above-mentioned ten phytohormones were detected under the following conditions:reversed-phase column Dikma C18 (250 mm×46 mm, 5 μm) at a temperature of 35℃, methanol/acetonitrile/phosphate buffer (pH=3.0) (20:20:60, vol/vol/vol) mixture as the mobile phase with a flow rate of 1 mL/min and a determination wavelength of 210 and 254 nanometer.The standard calibration curves of the ten phytohormones all showed a high linearity, the correlation coefficients being above 0.991 5.Their limits of detection ranged from 0.004 to 4.92 ng, with a relatively high precision (%RSD=0.026%-3.05%) and accuracy (%recovery=58.6%-85.2%).Then, this method was used to monitor the changes of phytohormone contents during the development of loquat fruitlet, and Lycopersicon esculentum and Brassica oleracea young leaves, and the results were satisfactory, suggesting that this method is an efficient tool for routine screening of phytohormone contents in various plants
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