|
|
- 2018
白魔芋开花过程内源激素动态变化
|
Abstract:
为探讨白魔芋开花过程中激素与雌、雄蕊成熟时间的关系，利用酶联免疫吸附法（ELISA）对白魔芋从佛焰苞形成前期到开花后期6个不同时期雄蕊和雌蕊中4种内源激素：生长素（IAA）、赤霉素（GA3）、脱落酸（ABA）和玉米素（ZT）的质量比进行测定和分析.结果表明，IAA，GA3，ABA和ZT 4种激素的质量比均呈现出不同的变化规律，IAA在雄蕊和雌蕊中均表现为下降-上升-下降的变化趋势；ABA表现出上升-下降-上升的变化趋势，与IAA的趋势相反；GA3在整个过程中没有明显的变化规律，雄蕊在佛焰苞成形后期达到最大值，约为195 ng/g，而雌蕊GA3的质量比晚于雄蕊达到最大值，雌蕊在开花前期才达到最大值，约为188 ng/g；ZT质量比的变化为先升后降、再升再降.在花芽形态分化，即雌、雄蕊成熟的关键时期，雌蕊中内源IAA，GA3和ZT的质量比均高于雄蕊，且GA3表现明显，说明较高浓度的IAA，GA3和ZT在成熟过程中起促进作用，在同一过程中，雄蕊中ABA的质量比却高于雌蕊的，而雄蕊晚熟于雌蕊，表明较高浓度的ABA抑制雄蕊的成熟.ABA/IAA和ABA/ZT的比值呈现出与ABA相同的变化趋势，高浓度的ABA/IAA，ABA/ZT和ABA/GA3均不利于雄蕊的成熟，进一步证明高浓度ABA是抑制雄蕊成熟的关键因子.
The aim of this study was to explore the relationship between hormones and pistil and stamen maturation during the flowering period of Amorphophallus albus. Four endogenous hormones, auxin (IAA), gibberellin (GA3), abscisic acid (ABA) and zeatin (ZT), in the pistils and the stamens in six different periods from the early stage of spathe formation to the late flowering period were measured and analyzed with enzyme-linked immunosorbent assay (ELISA). The experimental results showed that IAA, GA3, ABA and ZT showed different changes. IAA showed a trend of descending-ascending-descending in the stamens and the pistils and, to the contrary, ABA showed a trend of ascending-descending-ascending. GA3 content showed no distinct change pattern in the whole process of flowering, reaching its maximum in the late spathe formation in the stemens, about 195 ng/g; while GA3 content in the pistils reached the maximum later-in the first flowering, about 188 ng/g. A trend of ascending-descending-ascending-descending was recorded in ZT content in the stamens and the pistils. In the critical period of pistil and stamen maturation, the contents of endogenous IAA, GA3 and ZT in the pistils were higher than those in the stamens, which suggested that high concentrations of IAA, GA3 and ZT played a promoting role in the maturation process. In the same process, the content of ABA in the stamens was higher than that in the pistils, and the stamens matured slightly later than the pistils, which indicated that a higher concentration of ABA inhibited stamen maturation. The ratio of ABA/IAA and ABA/ZT showed the same trend as ABA, High concentration of ABA/IAA, ABA/ZT and
| [1] | 张上隆, 陈昆松, 叶庆富, 等. 柑桔授粉处理和单性结实子房(幼果)内源IAA、ABA和ZT含量的变化[J]. 园艺学报, 1994, 21(2): 117-123. |
| [2] | 吕忠恕. 果树生理[M]. 上海: 上海科学技术出版社, 1982. |
| [3] | 郑国华, 杉浦明, 米森敬三. 柿(Diospy ros kaki L.)果实发育及成熟过程中内源GA活性和ABA含量的变化[J]. 北京农业大学学报, 1991, 17(1): 77-82. |
| [4] | 牛自勉, 王贤萍, 李全, 等. 苹果花期前后子房内源激素的变化[J]. 园艺学报, 1996, 23(3): 291-292. |
| [5] | 丁静, 沈镇德, 方亦雄, 等. 植物内源激素的提取分离和生物鉴定[J]. 植物生理学通讯, 1979(2): 27-39, 50. |
| [6] | 张有林, 党娅, 张静, 等. 高效液相色谱法同时测定银凤桃中的赤霉素和脱落酸[J]. 西北植物学报, 2005, 25(7): 1467-1471. |
| [7] | 刘艳琴, 王浩, 杨红梅, 等. 高效液相色谱-串联四极杆质谱联用测定水果和蔬菜中赤霉素残留量[J]. 现代农业科技, 2009(16): 330-331. DOI:10.3969/j.issn.1007-5739.2009.16.231 |
| [8] | FORCAT S, BENNETT M H, MANSFIELD J W, et al. A Rapid and Robust Method for Simultaneously Measuring Changes in the Phytohormones ABA, JA and SA in Plants Following Biotic and Abiotic Stress[J]. Plant Methods, 2008, 4(1): 1-8. |
| [9] | 杨途熙, 魏安智, 郑元, 等. 高效液相色谱法同时分离测定仁用杏花芽中8种植物激素[J]. 分析化学, 2007, 35(9): 1359-1361. |
| [10] | MüLLER A, DüCHTING P, WEILER E W. A Multiplex GC-MS/MS Technique for the Sensitive and Quantitative Single-Run Analysis of Acidic Phytohormones and Related Compounds, and Its Application to Arabidopsis Thaliana[J]. Planta, 2002, 216(1): 44-56. |
| [11] | 金幼菊. 气相色谱-质谱联用技术在植物激素分析中的应用[J]. 植物生理学通讯, 1992, 28(1): 72-77. |
| [12] | 王平, 王海霞, 马英姿, 等. 蚬壳花椒叶片不定芽诱导与内源激素的变化规律[J]. 中草药, 2008, 39(9): 1400-1403. |
| [13] | 许欢, 马英姿, 曾慧杰, 等. 紫薇花器官分化过程内源激素变化研究[J]. 广东农业科学, 2015, 42(2): 37-42. |
| [14] | 林玲, 黄羽, 谢太理, 等. 3个葡萄品种花芽分化过程中内源激素含量变化初报[J]. 南方农业学报, 2012, 43(6): 806-809. |
| [15] | 曾骧. 果树生理学[M]. 北京: 北京农业大学出版社, 1992. |
| [16] | 宗学凤, 李芳红, 张建奎, 等. 蓝粒小麦植株发育进程中内源激素的变化[J]. 西南大学学报(自然科学版), 2007, 29(12): 58-62. |
| [17] | SEELEY S D, DAMAVANDY H, ANDERSON L M, et al. Autumn-Applied Growth Regulators Influence Leaf Retention, Bud Hardiness, Bud and Flower Size, and Endodormancy in Peach and Cherry[J]. Journal of the American Society for Horticultural Science American Society for Horticultural Science, 1992, 117(2): 203-208. |
| [18] | 苏华, 徐坤, 刘伟. 大葱花芽分化过程中内源激素的变化[J]. 园艺学报, 2007, 34(3): 671-676. |
| [19] | NU S R, CHEN W F, ZHOU X. Enzyme Linked Immunosorbent Assay for Endogenous Plant Hormones[J]. Plant Physiology Communications, 1988. |
| [20] | 王磊, 汤庚国, 刘彤. 石蒜花芽分化期内源激素和核酸含量的变化[J]. 南京林业大学学报(自然科学版), 2008, 32(4): 67-70. |
| [21] | 曹卫星, 王兆龙, 戴廷波. 不同穗型小麦小花发育过程中幼穗和叶片内源激素水平的动态变化[J]. 植物学报(英文版), 2000, 42(10): 1026-1032. DOI:10.3321/j.issn:1672-9072.2000.10.007 |
| [22] | 刘佩瑛. 魔芋学[M]. 北京: 中国农业出版社, 2004. |
| [23] | 吴志祥, 周兆德, 陶忠良, 等. 妃子笑与鹅蛋荔枝花芽分化期间内源激素的变化[J]. 热带作物学报, 2005, 26(4): 42-45. |
| [24] | BRIX H, PORTLOCK F T. Flowering Response of Western Hemlock Seedlings to Gibberellin and Water-Stress Treatments[J]. Canadian Journal of Forest Research, 1982, 12(1): 76-82. |
| [25] | 杨秋生, 籍越, 何松林, 等. 保鲜液对郁金香切花寿命和内源激素含量的影响(简报)[J]. 植物生理学通讯, 1996, 32(6): 416-419. |
| [26] | 林晓东. 激素调节花芽分化的研究进展[J]. 果树科学, 1997, 14(4): 269-274. |
| [27] | 芦站根. 燕子掌开花前后内源激素变化[J]. 中国园艺文摘, 2010, 26(6): 27-28. |
| [28] | 齐飞艳, 彭镇华, 高健, 等. 毛竹内源激素变化与开花特征相关性[C]//中国林学会竹子分会成立20周年暨中国竹业学术大会. 2012. |
| [29] | 曹尚银, 张俊昌, 魏立华. 苹果花芽孕育过程中内源激素的变化[J]. 果树科学, 2000, 17(4): 244-248. |
| [30] | 乔中全, 王晓明, 曾慧杰, 等. 不育紫薇'湘韵'扦插过程中内源激素含量变化[J]. 湖南林业科技, 2015, 42(1): 49-53. |
| [31] | 蔡坤秀, 牛先前, 王龙平, 等. 叶底红花芽分化期叶片内源激素的动态变化[J]. 福建农业学报, 2017, 32(3): 278-281. |
| [32] | WEILER E W. Immunoassay of Plant Growth Regulators[J]. Ann Rev Plant Physiol, 1984, 35(1): 85-95. |
| [33] | 马庆虎. 免疫学方法在植物激素研究中的应用[J]. 植物生理学通讯, 1988(4): 63-67. |
| [34] | 张亚芳, 何钢, 荣广天, 等. 鸡血藤愈伤组织培养过程中内源激素变化研究[J]. 生物技术通报, 2017, 33(3): 66-70. |
| [35] | 吕一帆, 张馨之, 晏姿, 等. 崇明水仙种球贮藏期内源激素变化规律[J]. 北方园艺, 2017(2): 76-79. |
| [36] | 李天红, 黄卫东, 孟昭清. 苹果花芽孕育机理的探讨[J]. 植物生理学报, 1996, 22(3): 251-257. |
| [37] | 史继孔, 张万萍, 樊卫国, 等. 银杏雌花芽分化过程中内源激素含量的变化[J]. 园艺学报, 1999, 26(3): 194-195. |
| [38] | 林荔仙, 张克平. 抗热大白菜加代育种技术[J]. 福建果树, 1992(1): 36. |
