青篱柴转录组的高通量测序及分析
High Throughput Sequencing and Analysis of Tirpitzia sinensis Transcriptome

青篱柴具有很好的观赏价值和药用价值，但其基因组信息相对缺乏，导致其分子生物学和基因功能的研究受到限制.为了获得和挖掘青篱柴的基因数据和功能，首次利用新一代高通量测序技术平台Illumina HiSeq^TM2000对青篱柴转录组进行测序，经de novo组装后共得到66 755条单基因簇(Unigene).进一步利用7大公共数据库进行Blast同源比对，注释了50 339条Unigene.研究发现，558个基因参与了青篱柴次生代谢产物的生物合成，其中在甜菜红碱、芥子油苷、类黄酮、单菌霉素和苯丙素生物合成途径中的Unigene分别有2个、18个、44个、70个和231个，这些基因很可能参与了青篱柴药用活性物质的生物合成. SSR位点搜索发现，在18 972条Unigene中含有22 542个SSR位点.研究结果将为青篱柴功能基因的克隆、基因的表达、分子标记开发和遗传多样性研究奠定基础.同时，转录因子分析结果也为青篱柴的喀斯特环境适应性机制提供了初步线索.
Tirpitzia sinensis has good value for ornamental and medicinal purposes, but its relative lack of genomic information has limited its research on molecular biology and gene function. In order to obtain and mining Tirpitzia sinensis genetic data and functionality, this study first used a new generation of high-throughput sequencing technology platform Illumina HiSeqTM2000 for Tirpitzia sinensis transcriptome sequencing, received 66 755 Unigene after De novo assembly. 50 339 Unigene were annotated by a similarity search against seven dadabases. The results showed that 558 genes were assigned to second metabolic pathway. Among them, 2 Unigenes were mapped to the betalain, 18 to glucosinolate, 44 to flavonoid, 70 to monobactam, 231 to phenylpropanoid biosynthesis pathways, These genes are likely to be involved in the biosynthesis of medicinal active substances in the Tirpitzia sinensis. A total of 22 542 SSRs were identified from the sequence of transcription, distributed in 18 972 Unigenes. The results will provide a basis for the cloning of gene, gene expression, molecular marker development and genetic diversity. Meanwhile, transcription factors prediction and analysis will provide preliminary insights into mechanisms of Tirpitzia sinensis for karst environmental adaptation