基于统计的基因定量技术圈定隐蔽油气藏有利区
Favorable Areas of Subtle Reservoirs Delineated by Statistics Based~Gene~Quantitative Technology

实时荧光PCR实现了功能基因定量，然而定量数据在油气藏，尤其是勘探难度大的隐蔽油气藏，定性预测方面的技术优势仍具开发潜力. 以勘探程度较低的苏干湖盆地隐蔽油气藏为对象，点间距14 km采集50 cm深土样191个，测定甲烷、丙烷氧化菌功能基因（ pmoA、 prmA），基于累积概率分布与回归分析，划定了微生物异常体系，中异常与物探地层解译结合，圈定了盆地油气勘探有利区. 结果显示： pmoA、 prmA累积概率分别符合近4^X 104、2^x 104 copies/g的指数分布规律，取自然数1、2、3、4分别以4、16、64、256和(2、4、8、16) 10copies/g划分二者异常的低、中、高、超高4等级；中异常以上等级于盆地东、西、中3部均有分布，其中，东、西分布稍偏北于二叠系、侏罗系地层，中部分布于二地层之间，保守圈定以上分布为盆地油气藏勘探有利区.
Functional genes could be qualitatively analyzed by real{{{\hyphen{}}}}time fluorescence PCR, but there is still much to learn from the qualitative data to qualitative reservoir prediction, especially great significance to the exploration of subtle reservoirs. Suganhu basin was studied in this work, which has the features of low exploration degree. 191 samples was collected with the interval of 1{{\hyphen{}}}4 km and the depth of 50 cm. Then the numbers of methane, propane oxidation bacteria functional genes ({\it pmoA}, {\it prmA}) in the samples were measured. Furthermore, based on the cumulative probability distribution and regression analysis, the anomaly grade classification system has been built, combing the anomaly zone with the strata interpreted by geophysical exploration, the favorable exploration areas of oil and gas in Suganhu basin were delineated. The results indicated that: the cumulative probability of {\it pmoA}, {\it prmA} nearly conform the 4\textsuperscript{{\it X}} $\times$ 10\textsuperscript{4}, 2\textsuperscript{{\it X}} $\times$ 10\textsuperscript{4} copies/g index distribution respectively, The natural numbers as 1, 2, 3, 4 were assigned to these two equations, thus obtained 4, 16, 64, 256 and (2, 4, 8, 16) $\times$ 10\textsuperscript{4} copies/g to divide the anomaly grades to four: low, medium, high and super. Anomaly zones above the medium level distribute in the east, west and central of basin, the medium anomaly zones in the east and west distribute slightly north of Permian and Jurassic strata, the medium anomaly zone in the central distribute between the two strata, these three medium anomaly zones above were delineated as the favorable exploration area of the~basin