毕赤酵母工程菌发酵生产重组Cu/Zn-SOD的工艺研究
Optimization of recombinant Cu/Zn-SOD production by Pichia pastoris in bench-top fermentors

系统考察毕赤酵母工程菌GS115表达重组铜锌超氧化物歧化酶（Cu/Zn-SOD）的发酵工艺参数，优化后5 L发酵罐上生产工艺为： 采用BSM培养基，接种量5%（体积分数），发酵温度35℃，诱导期搅拌转速900r·min-1，pH值为6.0、 维持溶氧（DO）水平为饱和值的20%以上. 经过142h发酵实验，SOD活力最高可达102.789kU·mL-1，比优化前提高3.5倍. 采用毕赤酵母工程菌能实现高效、 大量制备优质动物来源的Cu/Zn-SOD重组蛋白，具有良好的产业化潜力和广泛的应用前景.
In this study，detailed investigations were performed on effective production in 5L bench-top fermentor of a sika deer Cu/Zn-SOD by Pichia pastoris. The process parameters of recombinant SOD production were optimized to set up an appropriate fermentation procedure as follows: BSM medium，inoculum rate of 5%(volume fraction)，temperature of 35℃，agitation rate of 900r·min-1 during the induction stage，pH value of 6.0，DO level above 20% of the saturated value，and the cultivation period of 142h. Under the optimal conditions，the SOD activity in the broth could reach as high as 102.789kU·mL-1，which was increased by 3.5 folds. The results above established a highly effective fermentation procedure for industrial production of the recombinant sika deer Cu/Zn-SOD by Pichia pastoris that presented significant application prospect in future