单片式面膜中6种防腐剂的高效液相色谱法测定
Simultaneous determination of six preservatives in facial mask by high performance liquid chromatography

建立了同时测定单片式面膜中苯甲酸、山梨酸、脱氢乙酸、苯氧乙醇、氯苯甘醚和碘丙炔醇丁基氨甲酸酯6种防腐剂的高效液相色谱方法. 面膜经50%(体积分数）甲醇提取，采用C18色谱柱，以甲醇和乙酸铵溶液（0.02mol·L-1）为流动相进行梯度洗脱，流速为1.0mL·min-1，柱温30℃，苯甲酸、山梨酸、氯苯甘醚、苯氧乙醇和碘丙炔醇丁基氨甲酸酯的检测波长为235nm，脱氢乙酸的检测波长为295nm. 6种防腐剂浓度在0.5～20μg·mL-1范围内与峰面积均呈现良好的线性关系，方法的加标回收率为88.1%～105.3%，相对标准偏差为1.7%～2.8%. 方法操作简便、快速， 重复性好，灵敏度高，可用于面膜中6种防腐剂的测定.
The high performance liquid chromatographic method was developed for the simultaneous determination of six preservatives ( benzoic acid, sorbic acid, dehydro acetic, phenoxyethano, chlorphenesin, iodopropynyl butylcarbamate) in facial mask. The samples were extracted with 50% (volume fraction)methanol, and then separated on a C18 chromatographic column by gradient elution at a flow rate of 1.0mL·min-1 with methanol-ammonium acetate solution as the mobile phase. The column temperature was 30℃. The wavelength of detection for benzoic acid, sorbic acid, phenoxyethano, chlorphenesin, iodopropynyl butylcarbamate was 235nm, but for dehydroacetic acid was 295nm. In the range of 0.5 to 20μg·mL-1 the peak area of six preservatives showed a good linear relationship, the recoveries were 88.1% to 105.3% with the relative standard deviations 1.7% to 2.8%. The method is simple, rapid, accurate and sensitive, which can be used for determination of six preservatives in facial mask