响应面法优化洋金花中东莨菪碱的提取
Optimization on the extraction of scopolamine from the flower of Datura metel L. by response surface methodology

运用响应面法进行实验设计，对洋金花中东莨菪碱在超声提取过程中的各因素进行考察优化. 采用HPLC法测定东莨菪碱的质量浓度，以盐酸浓度、 提取时间、 液料比为考察因素，东莨菪碱提取率为响应值，用Box-Behnken响应面法设计提取工艺实验，建立预测方程，优化提取工艺. 结合实际可操作性，确定最佳提取条件为： 盐酸浓度3mol·L-1，提取时间43min，液料比25mL·g-1. 实验结果显示，实际值与预测值的偏差为2.76%，相关性良好，说明对洋金花中东莨菪碱的提取进行响应面法优化合理可行.
In this study，the extraction method of scopolamine from Datura metel L. was the ultrasonic extraction，while its mass concentration was detected by HPLC. Factors in the extraction process were investigated in a series of experiments designed by response surface methodology. Combined with the actual situation and the operable principles，the optimized conditions were as follows：the concentration of hydrochloric acid was 3mol·L-1，extraction time was 43min，and the ratio of liquid to solid was 25mL·g-1. The results displayed that the difference between the real value and the predicted value was 2.76%，showed good correlation，which indicating that the optimization on the extraction of scopolamine from the flower of Datura metel L. by response surface methodology was reasonable and practicable