猪生长激素受体(GHR)基因启动子的 克隆及功能分析
Characterization of Growth Hormone Receptor (GHR) Gene Promoter in pigs: Molecular Cloning and Functional Analysis

本研究以猪作为研究模型, 对GHR基因启动子区开展了克隆 、转录因子结合位点分析和启动子活性鉴定.结果表明: 家猪GHR基因或由两个启动子(GHR P1和GHR P2)控制.GHR P1的部分核苷酸序列与牛、羊对应核苷酸序列具有较高的同源性, 但功能分析显示其启动子活性较低.针对GHR P2的实验结果表明其具有典型的GHR组成型启动子特征, 荧光素酶报告实验表明其具有启动子活性, 因此我们推测本次克隆获得的GHR P2是猪GHR的组成型启动子.
The putative promoter regions of GHR were cloned by using pig as model. Furthermore, the transcription factor binding site was analyzed by using online software and promoter activities in cultured HepG2 cells. The results showed that partial nucleotide sequences of GHR P1 shared high homology with liver specific promoters of bovine and ovine, but its promoter activity is not obvious. Meanwhile, GHR P2 possess classical features of GHR constitutive promoter and dual luciferase reporting assay showed that GHR P2 have obvious promoter activity, these results indicate that GHR P2 cloned in this study is a constitutive promoter in pig