家猪STAT1的基因克隆、剪接变体鉴定及组织表达研究
Signal transducer and activator of transcription 1 gene (STAT1) in pig: cDNA cloning, tissue distribution, and identification of the alternative splicing variants

以长白猪(Landrace)cDNA为模板, 克隆STAT1基因cDNA全长.家猪STAT1基因cDNA全长编码757个氨基酸的前体蛋白, 与人、大鼠、小鼠、牛等哺乳动物的STAT1氨基酸序列一致性分别为95％、90？？8％、89？？6％、91？？6％; 同时, 本研究亦鉴定到家猪STAT1的3种剪接变体(分别命名为STAT1 sv1, STAT1 sv2, 和STAT1 sv3).采用RT PCR方法, 进一步检测了STAT1及其3种剪接变体在成年家猪各组织中的表达情况.STAT1和两种剪切变体(STAT1 sv2 和STAT1 sv3)在家猪组织中广泛表达, 而剪接变体STAT1 sv1则在肺和脾脏中有较高表达水平.
In this study, the full length cDNAs of pig STAT1 (pSTAT1) gene was cloned using RT PCR. The cloned pSTAT1 encodes a pro protein of 757 amino acids, which shares high amino acid sequence identity with that of human (95 %), mouse (89？？6 %), rat (90？？8%) and cattle (91？？6%). In addition, 3 novel mRNA splice variants of STAT1, named pSTAT1 sv1, pSTAT1 sv2 and pSTAT1 sv3 respectively, were identified in this study . RT PCR assay showed that STAT1 and 2 splice variants (pSTAT1 sv2 and pSTAT1 sv3) are expressed in all tissues examined, while pSTAT1 sv1 is abundantly expressed in spleen and Lung. These findings pave the way for further elucidation of the roles of STAT1 gene in pigs