拟南芥Hsp40蛋白AtDjA5在大肠杆菌中的功能分析
Functional analysis of the ？？Arabidopsis thaliana？？ Hsp40 protein AtDjA5 in ？？Escherichia coli？？

通过分析大肠杆菌？？dnaJ？？缺失菌株在43℃下的生长表型, 发现AtDjA5及其J Domain能够功能性地替换DnaJ及其J Domain. 免疫共沉淀实验结果表明AtDjA5与DnaK存在相互作用. 由Western blot检测推测AtDjA5能够稳定大肠杆菌热激转录因子σ？？？？32？？并下调热激蛋白DnaK表达量. 这些发现说明AtDjA5在大肠杆菌中具有与DnaJ相似的功能.
By analyzing growth phenotype of the ？？dnaJ ？？mutant strain at 43℃, AtDjA5 and its J Domain can functionally substitute for DnaJ and its J Domain. Co immunoprecipitation experiments show that AtDjA5 can interact with DnaK. It’s speculated that AtDjA5 can stabilize the heat shock factor σ？？？？32？？ of ？？E. coli？？ and down regulate the expression of Dnak by western blot analysis. These findings suggest that AtDjA5 has a similar function with DnaJ in ？？E. coli？？