Electrochemical Performances of Electroactive Nano-LayeredOrganic-Inorganic Perovskite Containing Trivalent Iron Ion and its Use for a DNA Biosensor Preparation
A steady nano organic-inorganic perovskite hybrid with Fe(CN)6 (3-AMP = 3-methylaminopyridine) was prepared in the air. The structure is an unusual layered organic-inorganic type. The resulting hybrid enveloped in paraffin to prepare Fe(CN)6 paste electrode (HPE) shows good electrochemical activity and a couple of oxidation and reduction peaks with potential of cyclic voltammometry (CV) at around 440？mV and 30？mV. Compared with that on CPE, oxidation potential of on HPE shifts negatively 259.7？mV and that of reduction shifts positively 338.7？mV, which exhibits that Fe(CN)6 can accelerate the electron-transfer to improve the electrochemical reaction reversibility. Such characteristics of Fe(CN)6 have been employed to prepare the DNA biosensor. The single-strand DNA (ssDNA) and double-strand DNA (dsDNA) immobilized on HPE, respectively, can improve the square wave voltammometry (SWV) current and SWV potential shifts positively. The effect of pH was evaluated. And there is hybridization peak on SWV curve using HPE immobilized ssDNA in the complementary ssDNA solution. And HPE immobilized ssDNA can be utilized to monitor the DNA hybridization and detect complementary ssDNA, covering range from to ？g/mL with detection limit of ？g/mL. The DNA biosensor exhibits a good stability and reproducibility. 1. Introduction In recent years, layered organic-inorganic perovskite hybrids have attracted great interest of researchers. Organic-inorganic perovskite hybrids offer an important opportunity to combine useful properties from two chemical realms, organic and inorganic compounds, within a single molecular scale composite. Especially, the design and generation of the organic-inorganic self-organized quantum well structure represent an approach to synthesis that offers new horizons in the context of synthetic chemistry and its possible impact on new material technology [1]. The hybrid organoammonium halometallate(II), (RNH3)2MX4, series of layer perovskite structures have played a significant role in the development of the understanding of low-dimensional magnetic systems and, more recently, of semiconducting, optical, photoelectric materials [2–6]. So far, only organic-inorganic perovskite hybrids containing the chemical element of tin (Sn), copper (Cu), and lead (Pb) have been used to application [7–13]. While it is not reported that organic-inorganic perovskite hybrids were used as electrochemical materials. On the other hand, a few organic-inorganic perovskite hybrids containing M3+ have been reported. As for conventional perovskite frameworks, trivalent metal
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