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- 2018
人颞下颌关节骨关节炎滑膜成纤维细胞中血管生成及润滑相关因子的表达
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Abstract:
摘要 目的: 探究血管生成相关因子及润滑相关因子在颞下颌关节骨关节炎滑膜中的表达。方法: 分离人颞下颌关节滑膜组织,组织块法进行滑膜成纤维细胞的原代及传代培养。流式细胞仪检测细胞中CD34及CD44的表达,Western blotting 和荧光定量 PCR分别用于检测VEGF 和 TSP1 蛋白, PRG4, HAS2和HAS3基因的表达。 结果: 骨关节炎滑膜细胞中VEGF、CD34和HAS3的表达显著高于对照组,但TSP1蛋白、PRG4和HAS2 基因的表达低于对照组。结论: 在颞下颌关节骨关节炎滑膜成纤维细胞中,血管生成相关因子高表达,而提高润滑作用的基因表达降低,该现象可能与该病的疾病发展有关
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