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- 2017
舌鳞状细胞癌淋巴结转移与长链非编码RNA HOTAIR的关系探讨
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Abstract:
摘要 目的:研究HOTAIR在舌鳞状细胞癌中的表达,并探究HOTAIR与舌鳞状细胞癌淋巴结转移的关系。方法:对19例舌鳞状细胞癌患者的癌组织以及其对应的19例癌旁组织进行实时荧光定量PCR检测,检测HOTAIR在癌组织、癌旁组织的相对表达量,把结果与患者的TNM分期进行关联比较,并进一步通过构建沉默HOTAIR表达的CAL27、SCC9细胞系,检测肿瘤细胞侵袭、迁移能力的改变。结果:19对舌鳞状细胞癌组织中,26.3%显示出HOTAI的高表达(P=0.016 9<0.05),且高表达者均为具有淋巴结转移的患者,而在无淋巴结转移的癌组织中HOTAIR呈现低表达(P<0.001),通过RNA干扰技术降低HOTAIR在CAL27及SCC9细胞系中的表达水平,结果发现细胞侵袭(P<0.001)和迁移能力(P<0.001)受到了抑制。结论:长链非编码RNA HOTAIR在舌鳞状细胞癌淋巴结转移过程中可能发挥促进因子的作用
| [1] | NIINUMA T, SUZUKI H, NOJIMA M, et al. Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors [J]. Cancer Res, 2012, 72(5)∶1126-1136 |
| [2] | 邹瑞,林颖,欧阳可雄,等. H19基因在肿瘤发生发展中作用的研究新进展[J]..实用医学杂志,2015,31(19)∶151-153 |
| [3] | Sun J, Chu H, Ji J, et al. Long non-coding RNA HOTAIR modulates HLA-G expression by absorbing miR-148a in human cervical cancer[J].Int J Oncol, 2016, 49(3)∶943-952 |
| [4] | Lee NK, Lee JH, Kim WK, et al. Promoter methylation of PCDH10 by HOTAIR regulates the progression of gastrointestinal stromal tumors[J].Oncotarget. 2016, 21. [Epub ahead of print] |
| [5] | Dhamija S, Diederichs S. From junk to master regulators of invasion: lncRNA functions in migration, EMT and metastasis [J]. Int J Cancer, 2016, 15, 139(2)∶269-280 |
| [6] | Li T, Mo X, Fu L, et al. Molecular mechanisms of longnoncoding RNAs on gastric cancer [J]. Oncotarget, 2016, 23(8)∶8601-8612 |
| [7] | ZHANG S, CHEN S, YANG G, et al. Long noncoding RNA HOTAIR as an independent prognostic marker in cancer: a meta-analysis [J]. PLoS One, 2014, 9(8)∶e105538 |
| [8] | American Joint Committee on Cancer. AJCC Cancer Staging Manual [M]. 7th Edition (2010). AJCC. 2009∶1-4 |
| [9] | 欧阳可雄,梁军,邹瑞,等.舌鳞癌组织长链非编码RNA的Ion Torrent高通量检测和分析[J]. 口腔疾病防治,2016,24(1)∶15-19 |
| [10] | ZENG XH, OU ZL, YU KD, et al. Absence of multiple atypical chemokine binders (ACBs) and the presence of VEGF and MMP-9 predict axillary lymph node metastasis in early breast carcinomas [J]. Med Oncol, 2014, 31(9)∶145-152 |
| [11] | KOGO R, SHIMAMURA T, MIMORI K, et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers [J]. Cancer Res, 2011, 71(20)∶6320-6326 |
| [12] | ISHIBASHI M, KOGO R, SHIBATA K, et al. Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma [J]. Oncol Rep, 2013, 29(3)∶946-950 |
| [13] | 付丛,张行炜.长链非编码RNA AFAP1-AS1在口腔鳞状细胞癌中的表达及临床意义[J].口腔医学研究,2016,32(9)∶983-986 |
| [14] | Lee M, Kim HJ, Kim SW, et al. The long non-coding RNA HOTAIR increases tumour growth and invasion in cervical cancer by targeting the Notch pathway [J].Oncotarget, 2016, 15(1)∶29-32 |
| [15] | WU Y, ZHANG L, WANG Y, et al. Long noncoding RNA HOTAIR involvement in cancer [J].Tumour Biol, 2014, 35(10)∶9531-9538 |
