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- 2017
干细胞因子在人慢性根尖周炎组织中表达的研究
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Abstract:
摘要 目的:观察干细胞因子(stem cell factor, SCF)在人慢性根尖周炎病变组织中成纤维细胞(fibroblasts, FBs)、内皮细胞(endothelial cells, ECs)及巨噬细胞(macrophages, m )上的表达,探讨SCF阳性的FBs、ECs及m 在人慢性根尖周炎发病机制中的作用。方法:本实验共收集50例根尖周组织标本,其中根尖周肉芽肿组15例,根尖周囊肿组15例,正常对照组20例。组织标本经福尔马林液固定,石蜡包埋、制作连续切片。HE染色,光学显微镜下观察各组标本的组织学变化;经免疫荧光双染色(double immunofluorescence, DIF),在荧光显微镜下观察SCF在根尖周组织FBs、ECs及m 上表达的情况。结果:DIF结果显示,两组慢性根尖周炎组织中的CD334-SCF双阳性FBs、CD31-SCF双阳性ECs及CD14-SCF双阳性m 密度较正常对照组显著增多(P＜0.01);根尖周囊肿组与根尖周肉芽肿组的CD334-SCF双阳性FBs及CD31-SCF双阳性ECs密度无显著性差异;根尖周肉芽肿组CD14-SCF双阳性m 密度明显高于根尖周囊肿组(P＜0.01)。结论:结果提示CD334-SCF双阳性FBs、CD31-SCF双阳性ECs及CD14-SCF双阳性m 与慢性根尖周炎的发生、发展及致病机制有关
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