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- 2017
种植体穿龈部分氮化钛纳米涂层对牙龈成纤维细胞粘附和增殖的影响
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Abstract:
摘要 目的:明确氮化钛纳米(TiN)涂层对人牙龈成纤维细胞(HGF)粘附形态和增殖的影响, 作为选择合适的种植体穿龈部分涂层的依据之一。方法:利用磁控溅射技术制作氮化钛(TiN)和对照Ti涂层,测量各组材料表面形貌、元素含量、粗糙度和亲水性,原代培养人牙龈成纤维细胞(HGF),通过扫描电镜SEM和CCK-8观察分析不同纳米涂层上方细胞粘附和增殖差异。结果:TiN组表面HGF粘附形态与Ti组相似,而HGF增殖幅度大于对照Ti。结论:本实验所设计的氮化钛(TiN)涂层利于促进HGF增殖,推荐作为种植体穿龈部分涂层
| [1] | 赖颖真,陈江.微沟槽形貌对人牙龈成纤维细胞粘附形态及增殖的影响[J].实用口腔医学,2015,31(4)∶515-519 |
| [2] | Chien CC, Liu KT, Duh JG, et al. Effect of nitride film coatings on cell compatibility[J]. Dent Mater, 2008, 24(7)∶986-993 |
| [3] | Annunziata M, Oliva A, Basile MA, et al. The effects of titanium nitride-coating on the topographic and biological features of TPS implant surfaces[J]. J Dent, 2011, 39(11)∶720-728 |
| [4] | Zhao G, Raines AL, Wieland M, et al. Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography[J]. Biomaterials, 2007, 28(18)∶2821-2829 |
| [5] | Yamano S, Ma AK, Shanti RM, et al. The influence of different implant materials on human gingival fibroblast morphology, proliferation, and gene expression[J]. Int J Oral Maxillofac Implants, 2011, 26(6)∶1247-1255 |
| [6] | Gr ssner-Schreiber B, Herzog M, Hedderich J, et al. Focal adhesion contact formation by fibroblasts cultured on surface-modified dental implants: an in vitro study [J]. Clin Oral Implants Res, 2006, 17(6)∶736-745 |
| [7] | Cyster LA, Parker KG, Parker TL, et al. The effect of surface chemistry and nanotopography of titanium nitride (TiN) films on 3T3-L1 fibroblasts[J]. J Biomed Mater Res A, 2003 67(1)∶138-147 |
| [8] | Balaban NQ, Schwarz US, Riveline D, et al. Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates[J]. Nat Cell Biol, 2001, 3(5)∶466-472 |
| [9] | Keselowsky BG, Collard DM, Garcia AJ. Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding[J]. Biomaterials, 2004, 25(28)∶5947-5954 |
| [10] | Ismail FS, Rohanizadeh R, Atwa S, et al. The influence of surface chemistry and topography on the contact guidance of MG63 osteoblast cells[J]. J Mater Sci Mater Med, 2007, 18(5)∶705-714 |
| [11] | Jin S, Zhang Y, Wang Q, et al. Influence of TiN coating on the biocompatibili-ty of medical NiTi alloy[J]. Colloids Surf B Biointerfaces, 2013, 101(2)∶343-349 |
| [12] | Cyster LA, Grant DM, Parker KG, et al. The effect of surface chemistry and structure of titanium nitride (TiN) films on primary hippocampal cells[J]. Biomol Eng, 2002, 19(2-6)∶171-175 |
