|
|
- 2016
载辛伐他汀羟基磷灰石中空微球的制备、表征及其用于盖髓剂的实验研究
|
Abstract:
摘要 目的: 制备羟基磷灰石中空微球并载辛伐他汀,构成体外药物缓释系统检验其对损伤大鼠牙髓的修复作用。方法: 以谷氨酸(Glu)为有机质,以十二烷基磺酸钠(SDS)和Glu组成的“核-壳”式复合物作为模板,制备出中空羟基磷灰石(HHAp)微球。表征产物形貌并载药,计算载药率和包封率,测定药物体外释放情况。雄性大鼠建立上颌第一磨牙直接盖髓模型。左侧上颌第一磨牙分别覆盖含有10-5 mol/L ,10-7 mol/L和10-9 mol/L 辛伐他汀的载药羟基磷灰石中空微球。第1组右侧上颌第一磨牙用未载药的HHAp微球盖髓,其余不作处理。分别于术后7 d、28 d,每组随机处死5只大鼠取材,HE染色。用图像分析软件INH测定各组标本中修复性牙本质的面积比例,并采用SPSS17.0对数据进行Dunnett t检验和配对t检验。结果: 合成的羟基磷灰石为由短针状纳米粒子组成的直径为2~4 μm中空的微球。载药率为21.3%~46.0%,包封率为34.46%~46.02%。药物在载药微球中缓慢释放,释放曲线接近线性。将含有不同浓度辛伐他汀的载药微球盖髓7 d后,对照组穿髓孔下方炎症反应明显,实验组则表现出轻微的炎症反应;盖髓后28 d, 10-7 mol/L SIM组与对照组、10-5 mol/L SIM组及10-9 mol/L SIM组相比形成了较多修复性牙本质(P<0.05)。结论: 利用小分子有机质(Glu)和表面活性剂(SDS)为模板可以合成中空羟基磷灰石微球,且此微球载药率和包封率均较高;药物在其中释放接近线性,达到缓释要求;含有10-7 mol/L SIM的载药微球能够显著促进牙髓损伤后修复性牙本质的形成
| [1] | Liu Y, Zhao X, Pan Y, et al. Facile Preparation of Hollow Hydroxyapatite Microspheres [J]. Acta Physico-Chimica Sinica, 2009, 25(7)∶1467-1471 |
| [2] | Okamoto Y, Sonoyama W, Ono M, et al. Simvastatin induces the odontogenic differentiation of human dental pulp stem cells in vitro and in vivo [J]. Journal of endodontics, 2009, 35(3)∶367-372 |
| [3] | Wu X, Zhao X, Li Y, et al. In situ synthesis carbonated hydroxyapatite layers on enamel slices with acidic amino acids by a novel two-step method [J]. Materials Science and Engineering: C, 2015, 54(1)∶150-157 |
| [4] | Pan Y, Zhao X, Guo Y, et al. Controlled synthesis of hollow calcite microspheres modulated by polyacrylic acid and sodium dodecyl sulfonate [J]. Materials Letters, 2007, 61(13)∶2810-2813 |
| [5] | 许莹莹,王蕊,郭冲冲,等.载辛伐他汀磷酸钙骨水泥/聚乳酸-聚羟基乙酸新型材料对拔牙创骨改建影响的实验研究[J].口腔医学研究,2011,27(11)∶954-956 |
| [6] | Yang Y H, Liu C H, Liang Y H, et al. Hollow mesoporous hydroxyapatite nanoparticles (hmHANPs) with enhanced drug loading and pH-responsive release properties for intracellular drug delivery [J]. Journal of Materials Chemistry B, 2013, 1(19)∶2447-2450 |
| [7] | Falini G, Albeck S, Weiner S, et al. Control of aragonite or calcite polymorphism by mollusk shell macromolecules [J]. Science, 1996, 271(5245)∶67-71 |
| [8] | Lang B R, Givan D A, Fitchie J G, et al. Tensile fatigue of 4-META cement bonding three base metal alloys to enamel and comparison to other resin cements [J]. The Journal of prosthetic dentistry, 1995, 73(4)∶377-385 |
| [9] | Lai E H H, Hong C Y, Kok S H, et al. Simvastatin alleviates the progression of periapical lesions by modulating autophagy and apoptosis in osteoblasts [J]. Journal of endodontics, 2012, 38(6)∶757-763 |
| [10] | Boonsongrit Y, Abe H, Sato K, et al. Controlled release of bovine serum albumin from hydroxyapatite microspheres for protein delivery system [J]. Materials Science and Engineering: B, 2008, 148(1)∶162-165 |
