|
|
- 2016
天然衍生脱细胞牛心包复合BMP-2转染的BMSCs引导骨组织再生的体外实验研究
|
Abstract:
摘要 目的: 研究天然衍生脱细胞牛心包复合BMP-2转染BMSCs引导骨组织再生的可行性和有效性。方法: 天然衍生脱细胞牛心包体外复合BMP-2转染BMSCs,SEM评估细胞生长与增殖,MTT法检测牛心包对转染细胞的毒性,ELISA 检测BMP-2含量,结晶紫染色法测定BMSCs细胞粘附能力,ALP活性实验检测转染BMSCs成骨性能。结果: SEM发现天然衍生脱细胞牛心包复合BMP-2基因转染的BMSCs后,细胞生长并增殖良好;各组细胞的存活率没有显著性差异;天然衍生脱细胞牛心包增强了BMP-2转染BMSCs的细胞粘附能力和成骨细胞转化能力。结论: 天然衍生脱细胞牛心包复合BMP-2基因转染BMSCs后,具有良好的生物和细胞相容性,有望成为一种优良的天然GBR膜材料
| [1] | Cui ZK, Fan J, Kim S, et al . Delivery of siRNA via cationic Sterosomes to enhance osteogenic differentiation of mesenchymal stem cells [J]. J of controlled release: official journal of the Controlled Release Society, 2015, 21∶742-52 |
| [2] | Woo KM, Chen VJ, Jung HM, et al. Comparative evaluation of nanofibrous scaffolding for bone regeneration in critical-size calvarial defects [J]. Tissue Eng Part A, 2009, 15(8)∶2155-62 |
| [3] | Kotobuki N, Ioku K, Kawagoe D, et al. Observation of osteogenic differentiation caseade of living mesenchymal stem cells on transparent hydroxyapatite ceramics [J]. Biomaterials, 2005, 26(7)∶779-85 |
| [4] | 白明海,张婷婷,谢辉,等.以牛心包为来源的天然衍生引导骨组织再生膜制备方法的实验研究[J].口腔医学研究,2016,32(5)∶440-444 |
| [5] | Ayyildiz A,Celebi B,Akgul KT. et al. A comparison of free skin graft,fescia lata,alloderm,bovine pericardium and primary repair in urethrocutaneous fistulas without diversion: an experimental study [J]. Pediatr Surg Int, 2006, 22(10)∶809-814 |
| [6] | Liu SB, Hu PZ, Hou Y, et al. Recombinant human bone morphogenetie protein-2 promote sthe proliferation of mesenchylnal stem cells in vivo and in vitro [J]. Chin Med J (Engl), 2009, 122(7)∶839-43 |
| [7] | Hong L, Tabata Y, Yamamoto M, et al. Comparison o fbone regeneration in a Rabbit skull defect by recombinant human BMP-2 incorporated in biodegradable hydrogel and in solution [J]. J Biomater Sci Polym Ed, 1998, 9(9)∶1001-14 |
| [8] | 白明海,凌天牖.天然衍生脱细胞牛心包膜复合BMP-2基因转染的骨髓间充质干细胞(BMSCs)引导骨组织再生的实验研究[D].中南大学博士论文,2013-12-01 |
| [9] | Olmos-Zuiga JR , Jasso-Victoria R, Gaxiola-Gaxiola M, et al. Comparison of lyophilized glutaraldehyde-preserved bovine pericardium with different vascular prostheses for use as vocal cords implants: experimental study [J]. BioMed research international, 201, 35∶1862 |
| [10] | Ansari S, Freire M, Choi MG, et al Effects of the orientation of anti-BMP2 monoclonal antibody immobilized on scaffold in antibody-mediated osseous regeneration [J]. J of biomaterials applications, 2015, 30(5)∶558-67 |
| [11] | Donzelli E, Salvade A, Mimo P, et al. Mesenchymal stem cells cultured on a collagen scaffold:In vitro osteogenic differentiation [J]. Arch Oral Biol, 2007, 52(1)∶64-73 |
| [12] | Sumanasinghe RD, Bemaeki SH, Loboa EG. Osteogenic differentiation of human mesenchymal stem cells in collagen matriees:effect of uniaxial cyclic tensile strain on bone morphogenctic Protein(BMP-2) mRNA expression [J]. Tissue Eng, 2006, 12(12)∶3459-65 |
| [13] | Gardin C, Ricci S, Ferroni L, Guazzo R, et al . Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures [J]. PloS one, 2015, 10 (7)∶e01323-44 |
| [14] | Xue Y, Datunark S, Xing Z, et al. Growth and differentiation of bon emarrow stromal cells on biodegradable polymer scaffolds:an invitro study [J]. J Bio med Mater Res, 2010, 95(4)∶1244-51 |
