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- 2017
镁离子对人牙周韧带细胞体外成骨能力的影响
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Abstract:
摘要 目的:探究不同浓度Mg2+对hPDLCs成骨向分化的影响,为后续牙周组织再生实验选择合适的Mg2+注入浓度提供依据。方法:取P3-P5代hPDLCs于Mg2+浓度分别为0、10、15、25、35、50 mmol/L条件中常规培养,用CCK-8法检测hPDLCs增殖情况。成骨诱导后比较各组ALP活性差异及茜素红矿化结节着色情况,进行RT-qPCR检测成骨相关基因Runx2、ALP、Col1、OPN及Bglap的表达差异。采用SPSS16.0软件,运用单因素方差分析进行统计学分析。结果:10、15、25 mmol/L Mg2+浓度可促进细胞增殖并提高ALP活性,35、50 mmol/L Mg2+浓度抑制细胞增殖及ALP活性。结论:适宜浓度镁离子(0~25 mmol/L)可促进hPDLCs早期成骨分化并抑制矿化
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