|
|
- 2017
局部注射黄芪多糖对大鼠正畸牙保持阶段BMP-2表达的影响
|
Abstract:
摘要 目的:探讨局部注射黄芪多糖对大鼠正畸牙保持阶段牙周组织改建和骨形成蛋白2(BMP-2)表达的影响。方法:48只8周龄雄性Wistar大鼠随机分为8组,每组6只,即空白对照组(1组),阴性对照组(1组),实验组(6组)。利用50 g牵引力近中移动右侧上颌第一磨牙,建立大鼠正畸牙移动模型,加力21 d。实验组于拆除装置前1 d开始将10 mg/L黄芪多糖溶液局部注射于右上第一磨牙远中颊侧黏骨膜下,每1次/3 d,每次50 μL;阴性对照组每3 d注射等量生理盐水。分别于1、3、7、14、21、28 d后处死,测量正畸牙复发距离,采用HE染色观察牙周组织变化,免疫组化染色检测牙周组织中BMP-2的表达。结果:实验组牙周膜宽度保持较好,除1 d组外,相同时间的实验组复发距离小于对照组,差异有统计学意义(P＜0.05)。在整个保持阶段,对照组远中侧BMP-2表达强度逐渐减少,实验组远中侧BMP-2表达强度逐渐增大。除1 d组外,相同时间的实验组BMP-2表达强度显著高于对照组,差异有统计学意义(P＜0.05)。结论:大鼠正畸牙保持阶段,局部注射黄芪多糖能够增加牙周组织中BMP-2的表达,从而加速牙槽骨的改建,有利于正畸后牙齿的保持
| [1] | Bessa PC, Casal M, Reis RL. Bone morphogenetic proteins in tissue engineering: the road from laboratory to clinic, part II ( BMP delivery) [J]. J Tissue Eng Regen Med, 2008, 2(2/3)∶81-96 |
| [2] | 王庭祥,杨贺,钟丽英,等.黄芪多糖毒性试验研究[J].现代预防医学,2009,36(23)∶4518-4519 |
| [3] | Canalis E, Economides A N, Gazzero E. Bone morphogenetic protein, their antagonists, and the skeleton [J]. Endocr Rev, 2003, 24(2)∶218-235 |
| [4] | Li XF, Ionescu AM, Schwarz EM, et al. Smad6 is induced by BMP-2 and modulates chondrocyte differentiation [J]. J Orthop Res, 2003, 21(5)∶908-913 |
| [5] | Lee KS, Kim HJ, Li QL, et al. Runx2 is a common target of transforming growth factor β1 and bone morphogenetic protein 2, and cooperation between Runx2 and Smad5 induces osteoblast-specific gene expression in the pluripotent mesenchymal precursor cell line C2C12 [J]. Mol Cell Biol, 2000, 20(23)∶8783-8792 |
| [6] | 孔祥鹤,牛银波,王婷梅,等.黄芪多糖对大鼠原代成骨细胞的影响及其机制研究[J].中草药,2011,42(10)∶2065-2069 |
| [7] | 刘豫蓉,张朝良,孔祥丽,等.黄芪多糖对成骨细胞增殖分化和结构的影响[J].国际口腔医学杂志,2010,37(2)∶133-137 |
| [8] | 丁寅.正畸矫治后保持与复发以及消炎痛、丹参等对正畸牙齿移动影响的研究[D].第四军医大学,1992 |
| [9] | 韩光红,陈远萍,刘天悦,等.正畸牙齿复发后牙周组织改建的实验研究[J].国际口腔医学杂志,2010,26(4)∶508-511 |
| [10] | 丁晓华,庄金良,姜雨君,等.辛伐他汀对大鼠正畸牙保持阶段牙周组织中BMP-2表达的影响[J].口腔医学研究,2014,30(4)∶306-310 |
| [11] | Urist MR. Bone: formation by autoinduction [J]. Science, 1965, 150(3698)∶893-899 |
| [12] | Kim J, Yang HJ, Cho TH, et al. Enhanced regeneration of rabbit mandibular defects through a combined treatment of electrical stimulation and rhBMP- 2 application [J]. Med Biol Eng Compu, 2013, 51(12)∶1339-1348 |
| [13] | KC Nune, A Kumar. Interplay between self-assembled structure of bone morphogenetic protein-2 (BMP-2) and osteoblast functions in three-dimensional titanium alloy scaffolds: Stimulation of osteogenic activity [J]. Journal of Biomedical Materials Research, 2016, 104(2): 517-532 |
| [14] | 王婷,张金超,杨梦甦,等.抗骨质疏松症的单味中药及药用植物研究进展[J].中国中药杂志,2006,31(9)∶718-721 |
| [15] | Hassel S, Schmitt S, Hartung A, et al. Initiation of Smad-dependent and Smad-independent signaling via distinct BMP-receptor complexes [J]. J Bone Joint Surg Am, 2003, 85(3)∶44-51 |
