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- 2017
PMX205抗炎作用的体外研究
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Abstract:
摘要 目的: 探讨补体C5a受体拮抗剂PMX205的体外抗炎作用。方法: MTT法检测PMX205对小鼠巨噬细胞(RAW264.7)的毒性。RAW264.7与牙龈蛋白酶(gingipain,G)体外共培养模拟炎症环境,观察PMX205的抗炎效果。实验分为6组:阴性对照组、G组、G+PMX205(0.1、1、5、10 mg/L)组,24 h后qRT-PCR、ELISA法检测M1型巨噬细胞标志性细胞因子IL-6及TNF-α,M2型标志性细胞因子IL-10和TGF-β1;流式细胞术检测M1型标志性分子CD86及M2型标志性分子CD206。结果: MTT结果显示,PMX205对RAW264.7活力影响较小。qRT-PCR及ELISA结果显示,G组IL-6和TNF-α表达水平较阴性对照组增加;G+PMX205各浓度组的IL-6和TNF-α的表达水平较G组减少,TGF-β1和IL-10表达水平均较G组增加(P＜0.01或P<0.05)。流式细胞术结果显示,G组CD86阳性率增加,CD206阳性率下降,G+PMX205组较G组CD86下降,而CD206增加。结论: 在体外细胞模型实验中PMX205具有良好的抗炎作用
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