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- 2016
整合素α6对模拟微重力下人牙髓干细胞粘附能力的影响
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Abstract:
摘要 目的:探究整合素α6对模拟微重力下人牙髓干细胞(human dental pulp stem cells, hDPSCs)粘附能力的影响。方法:以PLGA支架为载体,将采用酶消化法培养的hDPSCs接种在PLGA支架上,分普通重力组和模拟微重力组培养72 h,Western blot检测整合素α6(Integrin α6)、整合素αv(Integrin αv)、整合素β1(Integrin β1)、粘着斑激酶(focal adhesion kinase,FAK)、磷酸化粘着斑激酶(phospho-FAK)的达;si-RNA转染hDPSCs,抑制整合素α6表达,DAPI荧光染色检测转染后hDPSCs在PLGA支架上的粘附数量,Western blot检测转染后hDPSCs中整合素β1、FAK、phospho-FAK的表达。结果:模拟微重力下, 整合素α6、phospho-FAK蛋白水平上调(P＜0.05),整合素αv、整合素β1和FAK的蛋白水平未见显著性差异;si-RNA转染的hDPSCs粘附能力、phospho-FAK蛋白水平均低于对照组(P＜0.05),整合素β1和FAK的蛋白水平未见显著性差异。结论:模拟微重力下,hDPSCs在PLGA支架上粘附能力增强可能与整合素α6及其下游信号分子FAK的表达水平上调相关
| [1] | Cerwinka WH, Sharp SM, Boyan BD, et al. Differentiation of human mesenchymal stem cell spheroids under microgravity conditions [J] Cell Regen (Lond) ,2012 Jun 28;1(1):2. doi: 10.1186/2045-9769-1-2 |
| [2] | Zhu Q, Safavi KE, Spangberg LS. Integrin expression in human dental pulp cells and their role in cell attachment on extracellular matrix proteins [J]. J Endod,1998,24(10)∶641-644 |
| [3] | Geiger B, Bershadsky A, Pankov R, et al. Transmembrane extracellular matrix cytoskeleton crosstalk [J]. Nat Rev Mol Cell Biol,2001,2(11)∶793-805 |
| [4] | Suh H. Tissue restoration, tissue engineering and regenerative medicine [J]. Yonsei Med J,2000,41(6)∶681-684 |
| [5] | L.He, S.Pan, Y.Li, et al. Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity [J]. Int Endod J, 2015 Feb 19. doi: 10.1111/iej.12441 |
| [6] | Plett PA, Frankovitz SM, Abonour R, et al. Proliferation of human hematopoirtic bone marrow cells in microgravity [J]. In Vitro Cell Dev Biol Anim, 2001,37(2)∶73-78 |
| [7] | Giancotti FG, Ruoslahti E. Integrin Signaling [J]. Science, 1999, 285(5430)∶1028-1032 |
| [8] | Takeda M, Magaki T, Okazaki T,et al.Effects of simulated microgravity on proliferation and chemosensitivity in malignant glioma cells [J]. Neurosci Lett, 2009,463(1)∶54-59 |
| [9] | Yun HM, Lee ES, Kim MJ, et al. Magnetic Nanocomposite Scaffold-Induced Stimulation of Migration and Odontogenesis of Human Dental Pulp Cells through Integrin Signaling Pathways [J]. PLoS One, 2015 Sep 18;10(9)∶e0138614. doi: 10.1371 |
| [10] | Teo BK, Wong ST, Lim CK, et al. Nanotopography modulates mechanotransduction of stem cells and induces differentiation through focal adhesion kinase [J]. ACS Nano, 2013, 25, 7(6)∶4785-4798 |
| [11] | Lwigale PY, Thurmond JE, Norton WN, et al.Simulated microgravity and hypergravity attenuate heart tissue [J]. Cells Tissues Organs, 2000, 167(2-3)∶171-183 |
| [12] | Guignandon A, Lafage-Proust MH , Usson Y, et al. Cell cycling determines integrin-mediated adhesion in osteoblastic ROS 17/2.8 cells exposed to space-related conditions [J]. FASEB J,2001,15(11)∶2036-2038 |
