|
|
- 2016
纯钛超声微弧氧化-壳聚糖/海藻酸钠自组装种植体的动物实验研究
|
Abstract:
摘要 目的: 采用层层自组装技术(Layer-by-Layer self-assembly technique)在纯钛(TA2)微弧氧化表面制备壳聚糖(CS)/海藻酸钠(ALG)复合涂层,研究对骨结合的影响。方法: 选取24只新西兰大白兔建立双侧下颌骨种植模型,分别植入A、B、C组,TA2-MAO为A组,TA2-MAO-NAOH-CS为B组,TA2-MAO-NAOH-CS-ALG为C组。术后分别于2、4、6周取材,采用锥形束CT(cone beam,CBCT)和种植体骨扭出力测试的方法,观察种植体植入后骨结合的情况。选扭出6周的种植体做扫描电镜(SEM), 观察种植体涂层表面形貌。结果: CBCT观察:在2、4、6周,C组骨缺损区的愈合情况明显优于A、B组,B组骨缺损区的愈合情况稍好于A组,在6周时,观察到C组骨愈合良好,种植体骨界面无明显阴影区域,骨密度与正常骨密度一致。种植体骨扭出力测试中观察:在第2周时,A、B、C组的扭出力差异不明显,第4周时,B、C组扭力值高于A组,第6周时,C组的扭出力明显大于A、B组。在6周时,扫描电镜可以观察到c组对骨结合有促进作用。结论: TA2-MAO-NAOH-CS涂层和TA2-MAO-NAOH-CS-ALG涂层对骨结合都有促进作用,而TA2-MAO-NAOH-CS-ALG复合涂层对骨结合效果更好,双侧建立动物模型的方法可行
| [1] | Decher G. Fuzzy nanoassemblies: toward layered polymeric multicomposites [J]. Science,1997, 277(5)∶1232-1237 |
| [2] | Black J Push-out test [J]. J B joined Mater Res,1989,23(3)∶1243-1245 |
| [3] | Dhert WJ, Verheyen CC, Braak LH, et al. A finite element analysis of the push-out test influence of test conditions [J]. J B joined Mater Res,1992,26(1)∶119-130 |
| [4] | Morraa M, Cassinellia C, Carpib A, et al. EffeCS of molecular weight and surface functionalization on surface composition and cell adhesion to Hyaluronan coatedtitanium [J]. Biomed Pharmacother, 2006, 60(8)∶365-369 |
| [5] | Yamamoto O, Alvarez K, Kikuchi T, et al. Fabrication and characterization of oxygen-diffused titanium for biomedical applications [J]. Acta Biomater, 2009, 5(9)∶3605-3615 |
| [6] | Decher G, Hong JD. Buildup of ultrathin multilayer films by a self-assembly process. I.Consecutive adsorption of anionic and cationic bipolar amphiphiles on charged surfaces [J]. Makromol Chem Marcromol symp, 1991, 46(2)∶321-327 |
| [7] | 孟玲娜,沈兰花,王鑫,等.兔下颌骨种植体即刻种植动物模型的建立[J].中国口腔种植学杂志,2015,10(1)∶4-10 |
| [8] | 孙明霞,赵华强,刘少华,等.兔下颌骨牵张成骨模型的建立[J].口腔医学研究,2006,26(6)∶427-429 |
| [9] | 封伟,赵宝红,武金明,等.兔下颌骨双侧种植体植入动物模型的建立[J].口腔医学研究,2015,31(5)∶393-396 |
| [10] | Durn A, Conde A, Gómez Coedo A, et al. Sol-gel coatings for protection and bioactivation of metals used in orthopaedic devices [J]. J Mater Chem, 2004, 14(8)∶2282-2290 |
| [11] | 吴大怡.微创种植新概念.口腔种植骨量不足治疗技术与方案研讨会[J].西安会议,2014,10∶25-26 |
| [12] | S Hermann,D Buser,RK Schenk et al.Bone apposition around two different sandblasted and acid-etched titanium implant surfures :a histomorphometric study in canine mandibles [J]. Clin Oral Implants Res,2008,19(3)∶233-241 |
| [13] | Wennerberg A,S Galli, T Albrektsson. Current knowledge about the hydrophilic and nanostructured SLActive surface [J]. Clinical,Cosmetic and Investigational Dentistry,2011,3(1)∶59-67 |
| [14] | 陈夕.壳聚糖在水处理应用的研究进展[J].贵州化工,2010,35(4)∶33-36 |
| [15] | Motoyoshi M, Matsuoka M, Shimizu N. Application of orthodontic mini-implants in adolescents [J]. Int J Oral Maxillofac Surg, 2007, 36(8)∶695-9 |
