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- 2018
长链非编码RNA HOTAIR对舌鳞状细胞癌增殖及迁移侵袭能力的影响
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Abstract:
摘要 目的:探讨长链非编码RNA HOTAIR对舌鳞状细胞癌细胞系增殖及迁移侵袭能力的影响。方法:采用脂质体介导小干扰RNA(small interfering RNA, siRNA)敲低HOTAIR的表达,将无意义序列设为对照,采用定量反转录聚合酶链反应(quantificational revere transcriotion-polymerase chain reaction, qRT-PCR)检测沉默效果。噻唑蓝(methyl thiazol tetrazolium ,MTT)法检测各组细胞增殖能力的变化,划痕实验、Transwell实验检测各组细胞侵袭迁移能力的变化。结果:2株细胞系中HOTAIR成功敲低,差异具有统计学意义(P<0.001);在MTT实验中,与对照组比较,实验组细胞24、48、72、96 h时吸光度(A)值均降低(P<0.05);Transwell实验中实验组穿膜细胞数明显少于对照组(P<0.001),划痕实验中实验组的迁移距离少于对照组(P<0.001)。结论:HOTAIR促进了舌鳞状细胞癌细胞Tca-8113、TSCCA的增殖、迁移与侵袭,参与了舌癌的发展过程
| [1] | Ishibashi M,Kogo R,Shibata K,et al. Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma [J].Oncol Rep,2013,29(3):946-950 |
| [2] | Zhang Y, Zhang XO, Chen T, et al. Circular intronic long noncoding RNAs [J]. Mol Cell,2013,51(6):792-806 |
| [3] | Li DD, Feng JP, Wu TY, et al. Long intergenic noncoding RNA HOTAIR is overexpressed and regulates PTEN methylation in laryngeal squamous cell carcinoma [J]. Am J Pathol, 2013, 182(1):63-70 |
| [4] | Li J,Wang Y,Dong R,et al. HOTAIR enhanced aggressive biological behaviors and induced radio resistance via inhibiting p21 in cervical cancer [J]. Tumour Biol,2015,36(5):3611-3619 |
| [5] | Kung JT, Colognori D, Lee JT. Long noncoding RNAs: Past,present, and futures [J]. Genetics,2013, 193(3):651-669 |
| [6] | Nie Y,Liu X,Qu S,et al.Long non-coding RNA HOTAIR is an independent prognostic marker for nasopharyngeal carcinoma progression and survival [J]. Cancer Sci,2013,104(4):458-464 |
| [7] | Sorensen KP,Thomassen M,Tan Q,et al. Long non-coding RNA HOTAIR is an independent prognostic marker of metastasis in estrogen receptor positive primary breast cancer [J]. Breast Cancer Res Treat,2013,142(3):529-536 |
| [8] | Liu XH, Liu ZL, Sun M, et al. The long noncoding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non small cell lung cancer [J]. BMC Cancer, 2013, 13(1):464 |
| [9] | Ono H,Motoi H,Miyauchi E,et al. Long noncoding RNA HOTAIR is relevant to cellular proliferation, invasiveness, and clinical relapse in small-cell lung cancer [J]. Cancer Med,2014,3(3):632-642 |
| [10] | Torre LA,Bray F,Siegel RL,et al.Global cancer statistics,2012 [J]. CA cancer J Clin,2015,65(2):87-108 |
| [11] | Lee JT. Epigenetic regulation by long noncoding RNAs [J]. Science,2012,338(6113):1435-1439 |
| [12] | Chen FJ, Sun M, Li SQ, et al. Upregulation of the long noncoding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis [J]. Mol Carcinog,2013,52(11):908-915 |
| [13] | 蒋芳艳,王瑜,陈吉荣,等.转录因子XBP1在口腔鳞状细胞癌中的表达[J].口腔医学研究,2016,32(2):139-142 |
| [14] | Sun L,Fang J. Writer meets eraser in HOTAIR [J].Acta Biochim Biophys Sin(Shanghai) ,2011,43(1):1-3 |
| [15] | Kim K, Jutooru I, Chadalapaka G,et al. HOTAIR is a negative prognostic factor and exhibits prooncogenic activity in pancreatic cancer [J]. Oncogene,2013, 32(13):1616 -1625 |
| [16] | Tang L, Zhang W, Su B, et al.Long noncoding RNA HOTAIR is associated with motility, invasion, and metastatic potential of metastatic melanoma [J]. BioMed Res Int, 2013,2013:251098 |
| [17] | Bhan A, Hussain I, Ansari KI, et al. Bisphenol-A and diethylstilbestrol exposure induces the expression of breast cancer associated long noncoding RNA HOTAIR <i>in vitro</i> and <i>in vivo</i> [J]. J Steroid Biochem Mol Biol, 2014, 141(5):160-170 |
| [18] | He X, Bao W, Li X, et al. The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis [J]. Int J Mol Med 2014,33(2):325-332 |
