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- 2016
PEN载体传递ODN对人成骨样细胞细胞周期与成骨分化的影响
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Abstract:
摘要 目的: 采用新型载体PEN对ODN进行传递,探讨MT01/PEN复合物对人成骨样细胞(MG63)细胞周期及成骨分化的影响。方法: 选取不同装载比例的MT01/PEN复合物,以MT01及S-MT01做对照,分别检测其对MG63细胞周期及成骨分化相关基因ALP活性、BMP2、OCN表达的影响。结果: MT01/PEN组Gl期、G2期细胞百分比降低,S期比例增高(P<0.01),碱性磷酸酶活性增加(P<0.01),OCN、BMP2蛋白表达增高(P<0.05)。结论: 以PEN为载体传递MT01能促进MG63的分化
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