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- 2015
聚乳酸/羟基磷灰石(PLLA/HA)复合物预成乳前牙根管桩的生物相容性研究
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Abstract:
摘要 目的:评估聚乳酸/羟基磷灰石(PLLA/HA)复合物的生物相容性。方法:本实验主要参照我国医疗器械生物学评价标准,采用细胞黏附实验,溶血试验,急性毒性实验,热源实验和肌肉植入实验等系列体内外实验评价PLLA/HA的生物相容性。结果:L929细胞在材料表面生长良好;材料浸提液对实验兔不引起溶血反应、无致热源性;对小鼠无急性毒性;PLLA和PLLA/HA板块植入实验兔肌肉后,早期组织周围有轻微炎症反应,形成较厚纤维膜,随植入时间延长,炎症反应减轻,纤维包膜基本消失;SEM显示:初始纯PLLA结构致密,PLLA/HA中HA粒子大小均一,分布均匀,随降解时间延长,材料出现孔隙和裂纹。结论:PLLA/HA复合材料具有良好的生物相容性,符合生物安全性要求
| [1] | Lopes M S,Jardini A L,Maciel F R.Poly(lactic acid) production for tissue engineering applications [J]. ProcediaEngineering,2012,42∶1402-1413 |
| [2] | Mackool R, Yim J, McCarthy J G. Delayed degradation in a resorbable plating system [J]. J CraniofacSurg 2006, 17(1)∶194-197 |
| [3] | Liao SS, Cui F Z. In vitro and in vivo degradation of mineralized collagen-based composite scaffold: nanohydroxyapatite /collagen/poly( L-lactide) [J] Tissue Eng , 2004, 10(1-2)∶73-80 |
| [4] | Williams DF. On the biocompatibility of high technology materials [J]. Mat Res SocSymp Proc,1985,55∶117-125 |
| [5] | 杜欧,盖丽婷,王民艳,等.聚乳酸/羟基磷灰石复合材料的机械性能及其细胞毒性[J].吉林大学学报(医学版),2014,40(2)∶300-305 |
| [6] | Bayat M, Garajei A, GhorbaniK,et al. Treatment of mandibular angle fractures using a single bioresorbableminiplate [J]. J Oral Maxillofac Surg,2010,68(7)∶1573-1577 |
| [7] | Barrett D G, Yousaf M N. Design and applications of biodegradable polyester tissue scaffolds based on endogenous monomers found in human metabolism [J]. Molecules, 2009, 14(10)∶4022-4050 |
| [8] | Bayram B, Araz K, Uckan S, et al. Comparison of fixation stability of resorbable versus titanium plate and screws in mandibular angle fractures [J]. J Oral Maxillofac Surg,2009,67(8)∶1644-1648 |
| [9] | Grayson AC, VoskericianG, LynnA, et al. Differential degradation rates in vivo and in vitro of biocompatible poly(lactic acid) and poly(glycolic acid) homo-and co-polymers for a polymeric drug-delivery microchip [J]. J BiomaterSciPolym Ed,2004,15(10)∶1218-1304 |
| [10] | PihlajamkiH,BstmanO,TynninenO,et al. Long-term tissue response to bioabsorbable poly-L-lactide and metallic screws: An experimental study [J]. Bone,2006,39(4)∶932-937 |
| [11] | Williams DF. On the mechanisms of biocompatibility [J]. Biomaterials.2008,29(20)∶2941-2953 |
| [12] | Yerit K C , HainichS,Turhani D, et al. Stability of biodegradable implants in treatment of mandibular fractures [J]. PlastReconstrSurg, 2005, 115(7)∶1863-1870 |
| [13] | 郭晓东,郑启新,杜靖远,等.可吸收羟基磷灰石/ 聚DL-乳酸骨折内固定材料机械强度和生物降解性研究[J].中国生物医学工程学报, 2001,20(1)∶23-27 |
