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- 2016
NSCL/P患者中异常表达microRNA与WNT家族的关联性研究
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Abstract:
摘要 目的:探讨非综合征性唇腭裂患者中microRNA表达谱差异及显著改变microRNA与WNT家族的关联性。方法:采用涵盖当前miRbase数据库中所有人类microRNA的miRCURYTM探针的基因芯片对4例非综合征性唇腭裂患者的唇腭组织与4例正常新生儿脐带组织所提取的microRNA进行分析研究,并对差异性表达显著的microRNA进行生物信息学分析。结果:共筛选出254个与非综合征性唇腭裂相关的差异表达microRNA:181个在非综合征性唇腭裂组织中表达上调,73个表达下调(P＜0.05)。根据差异倍数与P值大小,选取十个表达差异较大的microRNAs进行生物信息学靶基因预测,结果显示hsa-miR-1260b, hsa-miR-205-5p, hsa-miR-24-3p, hsa-miR-27b-3p和 hsa-miR-720均靶向结合于Wnt5a、Wnt9b、Wnt10a等Wnt家族的信号分子。结论:差异表达较显著的microRNA通过与Wnt家族信号分子靶向结合参与调控唇腭裂的发育过程
| [1] | 王苹,王红,吴雅慧,等.中国人群叶酸/同型半胱氨酸代谢基因多态性与非综合征性唇腭裂相关研究[J].北京大学学报,2013,45(3)∶352-358 |
| [2] | Meng T, Shi B, Zheng Q, et al. Clinical and epidemiologic studies of nonsyndromic cleft lip and palate in china: analysis of 4268 cases [J]. Ann Plast Surg,2006,57(3)∶264-269 |
| [3] | 王鑫,赵尔杨.腭裂相关microRNA与信号通路的相互作用[J].口腔医学研究,2015,31(5)∶528-530 |
| [4] | He F, Xiong W, Yu X, et al.Wnt5a regulates directional cell migration and cell proliferation via Ror2-mediated noncanonical pathway in mammalian palate development [J]. Development,2008,135(23)∶3871-3879 |
| [5] | Lin C, Fisher AV, Yin Y, et al.The inductive role of Wnt-β-Catenin signaling in the formation of oral apparatus [J]. Dev Biol,2011,356(1)∶40-50 |
| [6] | Nishi M, Miyake H, Takeda T, et al. Congenital malformations and childhood cancer [J]. Med Pediatr Oncol,2000, 34(4)∶250-254 |
| [7] | Menezes R, Marazita ML, Goldstein McHenry T, et al. Axis inhibition protein 2, orofacial clefts and a family history of cancer [J]. J Am Dent Assoc,2009,140(1)∶80-84 |
| [8] | Siow MY, Ng LP, Vincent-Chong VK, et al.Dysregulation of miR-31 and miR-375 expression is associated with clinical outcomes in oral carcinoma [J]. Oral Dis,2014,20(4)∶345-351 |
| [9] | Cadigan KM, Nusse R. Wnt signaling: a common theme in animal development [J]. Genes Dev,1997,11(24)∶3286-3305 |
| [10] | Dale RM, Sisson BE, Topczewski J. The emerging role of Wnt/PCP signaling in organ formation [J]. Zebrafish,2009, 6(1)∶9-14 |
| [11] | Eberhart JK, He X, Swartz ME, et al. MicroRNA Mirn140 modulates Pdgf signaling during palatogenesis [J]. Nat Genet, 2008, 40(3)∶290-298 |
| [12] | Park MG, Kim JS, Park SY, et al. MicroRNA-27 promotes the differentiation of odontoblastic cell by targeting APC and activating Wnt/β-catenin signaling [J]. Gene,2014,538(2)∶266-272 |
| [13] | Zhu JL, Basso O, Hasle H, et al.Do parents of children with congenital malformations have a higher cancer risk? A nationwide study in Denmark [J]. Br J Cancer ,2002,87(5)∶524-528 |
| [14] | Andrade Filho PA, Letra A, Cramer A, et al. Insights from studies with oral cleft genes suggest associations between WNT-pathway genes and risk of oral cancer [J]. J Dent Res,2011,90(6)∶740-746 |
| [15] | 张萍. 非综合征性腭裂患者差异miRNA与靶基因分析及人心肌实时RT-PCR内参标志物在死亡早期稳定性的评估[D].上海,复旦大学,2012 |
| [16] | Rajatileka S, Luyt K, El-Bokle M, et al. Isolation of human genomic DNA for genetic analysis from premature neonates: a comparison between newborn dried blood spots, whole blood and umbilical cord tissue [J]. BMC Genet,2013,14∶105 |
| [17] | Warner DR, Mukhopadhyay P, Brock G, et al. MicroRNA expression profiling of the developing murine upper lip [J]. Dev Growth Differ, 2014, 56(6)∶434-447 |
| [18] | 蔡生青,王,石冰.WNT信号通路及其与唇腭裂发生[J].国际口腔医学杂志,2013,40(6)∶786-789 |
| [19] | Bejsovec A. Wnt pathway activation: new relations and locations [J]. Cell,2005,120(1)∶11-14 |
| [20] | Shin JO, Lee JM, Cho KW, et al. MiR-200b is involved in Tgf-β signaling to regulate mammalian palate development [J]. Histochem Cell Biol, 2012, 137(1)∶67-78 |
| [21] | Yu J, Chen Y, Qin L, et al.Effect of miR-205 on 3T3-L1 preadipocyte differentiation through targeting to glycogen synthase kinase 3 beta [J]. Biotechnol Lett,2014,36(6)∶1233-1243 |
| [22] | Listing H, Mardin WA, Wohlfromm S, et al. MiR-23a/-24-induced gene silencing results in mesothelial cell integration of pancreatic cancer [J]. Br J Cancer,2015,112(1)∶131-139 |
| [23] | Guo D, Li Q, Lv Q, et al. MiR-27a targets sFRP1 in hFOB cells to regulate proliferation, apoptosis and differentiation [J]. PLoS One,2014,9(3)∶e91354 |
| [24] | Bille C, Winther JF, Bautz A, et al.Cancer risk in persons with oral cleft: a population-based study of 8,093 cases [J]. Am J Epidemiol,2005,161(11)∶1047-1055 |
