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- 2015
卫星胶质细胞活化在束缚应激致大鼠咬肌痛觉敏感中的作用
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Abstract:
摘要 目的:观察慢性心理应激致咬肌痛觉敏感中三叉神经节卫星胶质细胞活化和P物质释放的关系。方法:40只健康雄性SD大鼠随机分为空白对照组、束缚应激3 d组、束缚应激7 d组、束缚应激14 d组。用体质量检测、旷场实验观察束缚应激后大鼠是否产生焦虑、抑郁样情绪;用Von Frey电子测痛仪检测大鼠咬肌痛阈。用免疫组织荧光染色法检测三叉神经节卫星胶质细胞活化的标志物神经胶质酸性蛋白(Glial fibrillary acidic protein, GFAP)及神经肽P物质(Substance P, SP)免疫阳性产物的变化。结果:受束缚应激后,大鼠体质量增长明显低于对照组,在旷场中总移动距离和总移动速度均明显降低,提示大鼠处于焦虑、抑郁样情绪状态。束缚应激后大鼠咬肌机械性痛阈明显降低;提示大鼠咬肌痛敏增强。束缚应激7 d、14 d组大鼠三叉神经节内GFAP及SP的表达明显高于空白对照组。结论:慢性心理应激可导致三叉神经节中的卫星胶质细胞活化,神经元SP表达升高。因此,三叉神经节中卫星胶质细胞和神经元的活化可能参与了慢性心理应激引起咬肌痛觉敏感升高过程
| [1] | Fried K, Bongenhielm U, Boissonade FM, et al. Nerve injury-induced pain in the trigeminal system [J]. The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry, 2001, 7(2)∶155-165 |
| [2] | Coelho SC, Bastos-Pereira AL, Fraga D, et al. Etanercept reduces thermal and mechanical orofacial hyperalgesia following inflammation and neuropathic injury [J]. European journal of pain, 2014, 18(7)∶957-967 |
| [3] | Takeda M, Tanimoto T, Kadoi J, et al. Enhanced excitability of nociceptive trigeminal ganglion neurons by satellite glial cytokine following peripheral inflammation [J]. Pain, 2007, 129(1-2)∶155-166 |
| [4] | Hanani M. Satellite glial cells in sensory ganglia: from form to function [J]. Brain research Brain research reviews, 2005, 48(3)∶457-476 |
| [5] | Chen WQ, Zhao XL, Hou Y, et al. Protective effects of green tea polyphenols on cognitive impairments induced by psychological stress in rats [J]. Behavioural brain research, 2009, 202(1)∶71-76 |
| [6] | Costa A, Smeraldi A, Tassorelli C, et al. Effects of acute and chronic restraint stress on nitroglycerin-induced hyperalgesia in rats [J]. Neuroscience letters, 2005, 383(1-2)∶7-11 |
| [7] | Grizzell JA, Iarkov A, Holmes R, et al. Cotinine reduces depressive-like behavior, working memory deficits, and synaptic loss associated with chronic stress in mice [J]. Behavioural brain research, 2014, 268∶55-65 |
| [8] | 张恒,周占松,刘丽梅,等. P物质对脊髓星形胶质细胞活化和炎性因子IL-1β、TNF-α分泌的影响[J].第三军医大学学报, 2006, 28(7)∶681-684 |
| [9] | Scrivani SJ, Keith DA, Kaban LB. Temporomandibular disorders [J]. The New England journal of medicine, 2008, 359(25)∶2693-2705 |
| [10] | Aggarwal VR, Macfarlane GJ, Farragher TM, et al. Risk factors for onset of chronic oro-facial pain--results of the North Cheshire oro-facial pain prospective population study [J]. Pain, 2010, 149(2)∶354-359 |
| [11] | Huang F, Zhang M, Chen YJ, et al. Psychological stress induces temporary masticatory muscle mechanical sensitivity in rats [J]. Journal of biomedicine & biotechnology, 2011, 2011∶720603 |
| [12] | Manfredini D, di Poggio AB, Romagnoli M, et al. Mood spectrum in patients with different painful temporomandibular disorders [J]. Cranio : the journal of craniomandibular practice, 2004, 22(3)∶234-240 |
| [13] | Rammelsberg P, LeResche L, Dworkin S, et al. Longitudinal outcome of temporomandibular disorders: a 5-year epidemiologic study of muscle disorders defined by research diagnostic criteria for temporomandibular disorders [J]. Journal of orofacial pain, 2003, 17(1)∶9-20 |
| [14] | 刘杨,张旻,赵雅娟,等.星形胶质细胞活化在心理应激影响大鼠咬肌痛觉敏感中的作用[J].牙体牙髓牙周病学杂志,2014, 24(5)∶258-263 |
| [15] | Matsuura S, Shimizu K, Shinoda M, et al. Mechanisms underlying ectopic persistent tooth-pulp pain following pulpal inflammation [J]. PloS one, 2013, 8(1)∶e52840 |
| [16] | Hidaka O, Yanagi M, Takada K. Changes in masseteric hemodynamics time-related to mental stress [J]. Journal of dental research, 2004, 83(2)∶185-190 |
| [17] | Fillingim RB, Ohrbach R, Greenspan JD, et al. Psychological factors associated with development of TMD: the OPPERA prospective cohort study [J]. The journal of pain : official journal of the American Pain Society, 2013, 14(12 Suppl)∶T75-90 |
| [18] | Chen YJ, Huang F, Zhang M, et al. Psychological stress alters ultrastructure and energy metabolism of masticatory muscle in rats [J]. Journal of biomedicine & biotechnology, 2010, 2010∶302693 |
| [19] | Li Q, Zhang M, Chen YJ, et al. Oxidative damage and HSP70 expression in masseter muscle induced by psychological stress in rats [J]. Physiology & behavior, 2011, 104(3)∶365-372 |
| [20] | Takeda M, Takahashi M, Matsumoto S. Contribution of activated interleukin receptors in trigeminal ganglion neurons to hyperalgesia via satellite glial interleukin-1beta paracrine mechanism [J]. Brain, behavior, and immunity, 2008, 22(7)∶1016-1023 |
| [21] | Zhao YJ, Li Q, Cheng BX, et al. Psychological stress delays periodontitis healing in rats: the involvement of basic fibroblast growth factor [J]. Mediators of inflammation, 2012, 2012∶732902 |
| [22] | Takayanagi Y, Fujita E, Yu Z, et al. Impairment of social and emotional behaviors in Cadm1-knockout mice [J]. Biochemical and biophysical research communications, 2010, 396(3)∶703-708 |
| [23] | Cao Y, Xie QF, Li K, et al. Experimental occlusal interference induces long-term masticatory muscle hyperalgesia in rats [J]. Pain, 2009, 144(3)∶287-293 |
| [24] | Gameiro GH, Andrade Ada S, de Castro M, et al. The effects of restraint stress on nociceptive responses induced by formalin injected in rat's TMJ [J]. Pharmacology, biochemistry, and behavior, 2005, 82(2)∶338-344 |
| [25] | List T, Axelsson S. Management of TMD: evidence from systematic reviews and meta-analyses [J]. Journal of oral rehabilitation, 2010, 37(6)∶430-451 |
| [26] | Jacobson-Pick S, Audet MC, McQuaid RJ, et al. Social agonistic distress in male and female mice: changes of behavior and brain monoamine functioning in relation to acute and chronic challenges [J]. PloS one, 2013, 8(4)∶e60133 |
| [27] | Couture R, Cuello AC. Studies on the trigeminal antidromic vasodilatation and plasma extravasation in the rat [J]. The Journal of physiology, 1984, 346∶273-285 |
| [28] | Takeda M, Tanimoto T, Nasu M, et al. Activation of NK1 receptor of trigeminal root ganglion via substance P paracrine mechanism contributes to the mechanical allodynia in the temporomandibular joint inflammation in rats [J]. Pain, 2005, 116(3)∶375-385 |
| [29] | McCarthy PW, Lawson SN. Cell type and conduction velocity of rat primary sensory neurons with calcitonin gene-related peptide-like immunoreactivity [J]. Neuroscience, 1990, 34(3)∶623-632 |
| [30] | Ambalavanar R, Moritani M, Moutanni A, et al. Deep tissue inflammation upregulates neuropeptides and evokes nociceptive behaviors which are modulated by a neuropeptide antagonist [J]. Pain, 2006, 120(1-2)∶53-68 |
