|
|
- 2016
常用分子检测技术在儿童龋病微生物多样性研究中的应用
|
Abstract:
摘要 龋病是口内常见的慢性、感染性疾病,在儿童中的发病率呈上升趋势。微生物是引起龋病的主要因素之一,对于致龋微生物的种类、致龋机制及其多样性是目前口腔微生物学的研究热点。随着现代分子生物学技术的不断发展,分子检测技术被广泛用于微生物的研究中,早期检测及预防对降低儿童龋病发病率有重要作用。本综述着重论述常用分子检测技术在儿童龋病微生物多样性研究方面的应用,并对未来儿童口腔微生物多样性方面的研究进行展望
| [1] | Qi X. The third national sampling epidemiological survey on oral health [M]. People’s Medical Publishing House: Beijing, 2008, pp∶72-76 |
| [2] | Chang RCC, So KF. Use of anti-aging herbal medicine, Lycium barbarum, against aging-associated diseases. What do we know so far [J]. Cellular and Molecular Neurobiology, 2008, 28(5)∶643-652 |
| [3] | Dewhirst FE, Chen T, Izard J, et al. The human oral microbiome[J]. Journal of bacteriology, 2010, 192(19)∶5002-5017 |
| [4] | 仵楠,赵今.白色念珠菌与主要致龋菌相关性研究[J]. 口腔医学研究, 2012, 28(010)∶1080-1082 |
| [5] | Becker MR, Paster BJ, Leys EJ, et al. Molecular analysis of bacterial species associated with childhood caries[J]. Journal of clinical microbiology, 2002, 40(3)∶1001-1009 |
| [6] | Tanner A C R, Mathney J M J, Kent R L, et al. Cultivable anaerobic microbiota of severe early childhood caries[J]. Journal of clinical microbiology, 2011, 49(4)∶1464-1474 |
| [7] | Corby PM, Lyons-Weiler J, Bretz WA, et al. Microbial risk indicators of early childhood caries[J]. Journal of clinical microbiology, 2005, 43(11)∶5753-5759 |
| [8] | Kanasi E, Dewhirst FE, Chalmers NI, et al. Clonal analysis of the microbiota of severe early childhood caries[J]. Caries research, 2010, 44(5)∶485-497 |
| [9] | Aas JA, Griffen AL, Dardis SR, et al. Bacteria of dental caries in primary and permanent teeth in children and young adults[J]. Journal of clinical microbiology, 2008, 46(4)∶1407-1417 |
| [10] | Nogueira RD, Sesso MLT, Borges MCL, et al. Salivary IgA antibody responses to Streptococcus mitis and Streptococcus mutans in preterm and fullterm newborn children[J]. Archives of oral biology, 2012, 57(6)∶647-653 |
| [11] | Gross EL, Leys EJ, Gasparovich SR, et al. Bacterial 16S sequence analysis of severe caries in young permanent teeth[J]. Journal of clinical microbiology, 2010, 48(11)∶4121-4128 |
| [12] | Parahitiyawa NB, Scully C, Leung WK, et al. Exploring the oral bacterial flora: current status and future directions[J]. Oral diseases, 2010, 16(2)∶136-145 |
| [13] | Bik EM, Long CD, Armitage GC, et al. Bacterial diversity in the oral cavity of 10 healthy individuals[J]. The ISME journal, 2010, 4(8)∶962-974 |
| [14] | Faveri M, Gon alves LFH, Feres M, et al. Prevalence and microbiological diversity of Archaea in peri‐implantitis subjects by 16S ribosomal RNA clonal analysis[J]. Journal of periodontal research, 2011, 46(3)∶338-344 |
| [15] | Schirrmeister JF, Liebenow AL, Pelz K, et al. New bacterial compositions in root-filled teeth with periradicular lesions[J]. Journal of endodontics, 2009, 35(2)∶169-174 |
| [16] | Subramanian K, Mickel AK. Molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile[J]. Journal of endodontics, 2009, 35(7)∶950-957 |
| [17] | Gross EL, Beall CJ, Kutsch SR, et al. Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis[J]. PloS one, 2012, 7(10)∶e47722 |
| [18] | Peterson SN, Snesrud E, Liu J, et al. The dental plaque microbiome in health and disease[J]. PloS one, 2013, 8(3)∶e58487 |
| [19] | Jumpstart Consortium Human Microbiome Project Data Generation Working Group. Evaluation of 16SrDNA-based community profiling for human microbiome research[J]. PLoS One, 2012, 7(6)∶e39315 |
| [20] | Luo P, Hu C, Zhang L, et al. Effects of DNA extraction and universal primers on 16S rRNA gene-based DGGE analysis of a bacterial community from fish farming water[J]. Chinese Journal of Oceanology and Limnology, 2007, 25∶310-316 |
| [21] | Crielaard W, Zaura E, Schuller A A, et al. Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health[J]. BMC medical genomics, 2011, 4(1)∶22 |
| [22] | Jiang W, Zhang J, Chen H. Pyrosequencing analysis of oral microbiota in children with severe early childhood dental caries[J]. Current microbiology, 2013, 67(5)∶537-542 |
| [23] | Belda-Ferre P, Alcaraz L D, Cabrera-Rubio R, et al. The oral metagenome in health and disease[J]. The ISME journal, 2011, 6(1)∶46-56 |
| [24] | Hamady M, Knight R. Microbial community profiling for human microbiome projects: Tools, techniques, and challenges[J]. Genome research, 2009, 19(7)∶1141-1152 |
| [25] | Kumar PS, Brooker MR, Dowd S E, et al. Target region selection is a critical determinant of community fingerprints generated by 16S pyrosequencing[J]. PloS one, 2011, 6(6)∶e20956 |
| [26] | Luo AH, Yang DQ, Xin BC, et al. Microbial profiles in saliva from children with and without caries in mixed dentition[J]. Oral diseases, 2012, 18(6)∶595-601 |
| [27] | Kanasi E, Johansson I, Lu SC, et a1. Microbial risk markers for childhood caries in pediatricians’offices[J]. J Dellt Res, 2010, 89(4)∶378-383 |
| [28] | 李艳莉. 微阵列分析龋易感儿童患龋前后牙菌斑生物膜微生物组成多样性[D]. 遵义医学院, 2009 |
| [29] | Li Y, Ge Y, Saxena D, et al. Genetic profiling of the oral microbiota associated with severe early-childhood caries[J]. Clinical Microbiology, 2007,45(1)∶81-87 |
| [30] | Ye Tao, Yan Zhou, Yong Ouyang, et al. Dynamics of oral microbial community profiling during severe early childhood caries development monitored by PCR-DGGE[J]. Archives of Oral Biology, 2013, 58:∶1129-1138 |
| [31] | 陆剑,相艳,李宇红,等.PCR-DGGE初步分析双胞胎儿童牙菌斑细菌群落组成[J]. 口腔医学研究, 2012, 28(010)∶997-999 |
| [32] | Kirk JL, Beaudettte LA, Hart M,et al. Methods of studying soil microbial diversity [J]. J Microbiol Methods, 2004, 58(3)∶169-188 |
| [33] | 刘震华,连冰洁,赵今.维吾尔族不同龋敏感儿童变形链球菌临床分离株质子转运 ATP 合酶亚基 cab 基因遗传多态性研究[J]. 中华口腔医学, 2012, 47(9)∶547-551 |
| [34] | Piwat S, Teanpaisan1 R, Thitasomakul S,et al. Lactobacillus species and genotypes associated with dental caries in Thai preschool children[J].Molecular Oral Microbiology, 2010, 25∶157-164 |
| [35] | Walter J, Psoter Yao, Stefanie Ge, et al. PCR detection of Streptococcus mutans and Aggregatibacter actinomycetemcomitans in dental plaque samples from Haitian adolescents. Clin Oral Invest, 2011, 15∶461-469 |
| [36] | Nurelhuda NM, Al-Haroni M, Trovik TA, et al. Caries experience and quantification of Streptococcus mutans and Streptococcus sobrinus in saliva of Sudanese schoolchildren[J]. Caries research, 2010, 44(4)∶402-407 |
| [37] | Nyvad B, Crielaard W, Mira A, et al. Dental caries from a molecular microbiological perspective[J]. Caries research, 2012, 47(2)∶89-102 |
| [38] | Ling Z, Kong J, Jia P, et al. Analysis of oral microbiota in children with dental caries by PCR-DGGE and barcoded pyrosequencing[J]. Microbial ecology, 2010, 60(3)∶677-690 |
| [39] | Yang R, Argimon S, Li Y, et al. Determining the genetic diversity of lactobacilli from the oral cavity[J]. Journal of microbiological methods, 2010, 82(2)∶163-169 |
