|
|
- 2016
两种沟槽钛片上纤维连接蛋白修饰方法的效果比较
|
Abstract:
摘要 目的:比较三氟代乙烷磺酰氯与硅烷化两种钛片上修饰纤维粘结蛋白(Fibronectin,Fn)方法的效果。方法:通过三氟代乙烷磺酰氯与硅烷化将Fn修饰于微沟槽钛表面,XPS分析不同材料表面元素组成;荧光染色观察接种不同材料表面的蛋白数量及分布。DAPI染色检测HGF接种在不同材料表面2、4、6 h后早期附着细胞数。用cck8比较HGF接种不同材料表面6 h、12 h、1 d、3 d、5 d、7 d后细胞增值率。结果:XPS结果提示硅烷化组材料表面的纤维蛋白含量高于三氟组及空白组(P<0.05)。免疫荧光观察提示硅烷化组材料表面的纤维连接蛋白含量高于三氟组及空白组(P<0.05)。DAPI染色提示2 h时3组材料细胞附着数相当,4、6 h时硅烷化组材料表面上细胞数明显多于其他组材料(P<0.05)。CCK8结果提示硅烷化材料在6个观察时间段硅烷化组材料细胞增值率高于三氟组及空白组(P<0.05)。结论:硅烷化法在钛片上修饰纤维蛋白的效果优于三氟代乙烷磺酰氯的方法
| [1] | Zhao BH, Cui FZ, Liu Y, et al. Histomorphometrical and clinical study of connective tissue around titanium dental implants with porous surfaces in a canine model [J]. J Biomater Appl, 2011, 27(6)∶685-693 |
| [2] | Botos S, Yousef H, Zweig B, et al. The Effects of Laser Microtexturing of the Dental Implant Collar on Crestal Bone Levels and Peri-implant Health [J]. Int J Oral Maxillofac Implants, 2011, 26(3)∶492-498 |
| [3] | Werner S, Huck O, Frisch B, et al. The effect of microstructured surfaces and laminin-derived peptide coatings on soft tissue interactions with titanium dental implants [J]. Biomaterials, 2009, 30(12)∶2291-2301 |
| [4] | Wei-Bor Tsai, Yen-Chung Ting, Jung-Yen Yang, et al. Fibronectin modulates the morphology of osteoblast-like cells (MG-63) on nano-grooved substrates [J]. Mater Med, 2009, 20(11)∶1367-1378 |
| [5] | 粱芳慧,周廉.钛和钛合金生物活化研究现状[J].稀有金属材料与工程,2003,32(4)∶241-245 |
| [6] | Guicai Li, Ping Yang, Xiang Guo, et al. Anin vitro evaluation of inflammation response of titanium functionalized with heparin/fibronectin complex [J]. Cytokine, 2011, 56(1)∶208-217 |
| [7] | Eiji Yoshida, Yoshitaka Yoshimura, Motohiro Uo, et al. Influence of nanometer smoothness and fibronectin mmobilizationof titanium surface on MC3T3-E1 cell behavior [J]. Published online 23 March 2012 in Wiley Online Library (wileyonlinelibrary.com). DOI: 10.1002/jbm.a.3408 |
| [8] | M Chimutengwende-Gordon, C Pendegrass and G Blunn. Enhancing the soft tissue seal around intraosseous transcutaneous amputation prostheses using silanized fibronectin titanium alloy [J]. Biochem. Mater, 2011, 36(6)∶1-11 |
| [9] | An N, Rausch-fan X, Wieland M, et al. Initial attachment, subsequent cell proliferation/viability and gene expression of epithelial cells related to attachment and wound healing in response to different titanium surfaces [J]. Dent Mater, 2012, 28(12)∶1207-1214 |
| [10] | HayakawaT, Yoshinari M, Nagai M, et al. X-ray Photoelectron Spectroscopic Studies of the Reactivity of Basic Terminal OH of Titanium towards Tresyl Chloride and Fibronectin [J]. Biomedical Res, 2003,24(5)∶223-230 |
| [11] | 黄斯佳,许志强,樊牮,等.纤维连接蛋白修饰的微沟槽钛片对人牙龈成纤维细胞活性影响的研究[J].口腔医学研究,2015,31(11)∶1168-1172 |
| [12] | Lai Y, Chen J, Zhang T, et al. Effect of 3D microgroove surface topography on plasma and cellular fibronectin of human gingival fibroblasts [J]. J. Dent, 2013,(41)∶1109-1121 |
