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- 2015
糖基化终末产物对人牙周膜干细胞增殖及相关基因HSG、cyclinD1表达的影响
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Abstract:
摘要 目的:探讨糖基化终末产物(AGEs)对人牙周膜干细胞(HPDLSC)增殖能力以及增殖相关基因HSG、cyclinD1的影响。方法:体外组织块法和有限稀释法克隆化培养牙周膜干细胞;成骨、成脂诱导牙周膜细胞,对其进行干细胞鉴定;将培养出的牙周膜干细胞与不同浓度的AGEs 共培养,MTT检测不同浓度下牙周膜干细胞增殖的改变;实时定量聚合酶链反应(real time PCR)检测AGEs刺激后HSG、cyclin D1表达的改变。结果:牙周膜细胞成骨诱导21 d后茜素红染色出现钙化结节;成脂诱导21 d后油红O染色出现脂滴;MTT显示不同浓度的AGEs对HPDLSCs增殖能力的影响有所差别,高浓度(100 mg/L,200 mg/L)明显抑制HPDLSCs的增殖,差异有统计学意义(P<0.05),低浓度(1 mg/L,10 mg/L)对HPDLSCs的增殖能力影响不大,差异无统计学意义;Real time PCR结果显示:AGEs(100 mg/L)刺激3 d后HSG mRNA表达水平较对照组升高、cyclinD1 mRNA的表达水平较对照组降低,差异有统计学意义(P<0.05)。结论:高浓度的AGEs能抑制人牙周膜干细胞的增殖并能改变HSG、cyclinD1 mRNA的表达
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