|
|
- 2015
导下颌向前对生长期大鼠髁突软骨结缔组织生长因子表达的影响
|
Abstract:
摘要 目的:通过建立导下颌向前的Sprague-Dawley (SD)大鼠的动物模型进行免疫组化实验,观察结缔组织生长因子(CTGF)在髁突软骨中分布的变化,探讨CTGF对前伸下颌后的髁突软骨的影响。方法:建立SD大鼠动物模型,选取45只5周龄SD 雌性大鼠,体重约100 g,随机分为对照组(20只)和实验组(25)只,组内再随机平均分为5组(3、7、14、21 和30 d)。实验组动物24 h佩戴改良可摘式上颌斜面导板矫治器,对照组动物不做处理。免疫组化染色标记CTGF,观察髁突软骨各层CTGF的表达变化。对CTGF的阳性表达量进行半定量分析。结果:对照组和实验组中髁突软骨组织中均有CTGF的表达,主要分布于肥大层和增殖层。实验组不同时间点髁突肥大层和增殖层中CTGF的平均光密度值分别高于对照组的平均光密度值(P<0.05 ) 。与对照组相比,实验组7 d后髁突肥大层和增殖层中CTGF的表达开始上升,到第14天达到最高,之后CTGF表达回落,但仍高于对照组(P<0.05 )。实验3 d、30 d,肥大层和增殖层中CTGF实验组与对照组相比无统计学差异。结论:CTGF可能参与了功能负荷改变所导致的髁突软骨适应性改建活动
| [1] | Rabie AB, She TT, Hagg U, et al. Functional appliance therapy accelerates and enhances condylar growth [J]. Am J Orthod Dentofacial Orthop, 2003, 123(1)∶40-48 |
| [2] | Song Y, Wu C, Wong R, et al. Identification of the chondrogenic pathway in the mandibular condylar cartilage [J]. Frontiers in Bioscience, 2009, 14(1)∶1932-1938 |
| [3] | Gupta A, Hazarey PV, Kharbanda OP, et al. Stress distribution in the temporomandibular joint after mandibular protraction: a 3-dimensional finite element method study. Part 2 [J]. Am J Orthod Dentofac Orthop, 2009, 135(6)∶749-756 |
| [4] | 王贺,张娟,尹硕,等.MMP-3诱导人牙髓成纤维细胞CCN2mRNA的表达及意义[J].口腔医学研究,2014,30(5)∶399-402 |
| [5] | Itoh S, Hattori T, Tomita N, et al. CCN Family Member 2/Connective Tissue Growth Factor (CCN2/CTGF) has anti-aging effects that protect articular cartilage from age-related degenerative changes [J]. Plos one, 2013, 8(8)∶1-15 |
| [6] | 华先明,程祥荣.下颌髁突软骨的结构和在拉伸/剪切负荷作用下的生物机械性能[J].口腔医学研究,2014,30(2)∶177-179 |
| [7] | Rabie AB, Wong L, Hagg U. Correlation of replicating cells and osteogenesis in the glenoid fossa during stepwise advancement [J]. Am J Orthod Dentofacial Orthop, 2003, 123(5)∶521-526 |
| [8] | Rabie AB, Xiong H, Hagg U. Forward mandibular positioning enhances condylar adaptation in adult rats [J]. Eur J Orthod, 2004, 26(4)∶353-358 |
| [9] | McNamara JA , Bryan FA. Long term mandibular adaptations to protrusive function : An experimental study in Macaca Mulatta [J]. Am J Orthod Dentofac Orthop, 1987, 92-98 |
| [10] | Rabie AB, Zhao Z, Shen G, et al. Osteogenesis in the glenoid fossa in response to mandibular advancement [J]. Am J Orthod Dentofacial Orthop, 2001, 119(4)∶390-400 |
| [11] | 白玉兴,罗颂椒.功能矫治前伸下颌后大鼠髁突软骨雌激素受体变化规律的研究[J].中华口腔医学杂志,1998,33(6)∶372-374 |
| [12] | Owtad P, Potres Z, Shen G, et al. A histochemical study on condylar cartilage and glenoid fossa during mandibular advancement [J]. Angle Orthod, 2011, 81(2)∶270-276 |
| [13] | Shen G, Rabie AB, Zhao ZH, et al. Forward deviation of the mandibular condyle enhances endochondral ossification of condylar cartilage indicated by increased expression of type X collagen [J]. Arch Oral Biol, 2006, 51(4)∶315-324 |
| [14] | Tomita N, Hattori T, Ito S, et al. Cartilage-specific overexpression of CCN family member 2/connective tissue growth factor (CCN2/CTGF) stimulates insulin-like growth factor expression and bone growth [J]. Plos One, 2013, 8∶59226 |
| [15] | Al-Kalaly AA, Dyson JE, Wong RW, et al. Effect of different advancement positions on the maximum retrusive force of the mandible [J]. Orthod Craniofac Res, 2013, 16(1)∶56-64 |
| [16] | Nishida T, Kubota S, Kojima S, et al. Regeneration of defects in articular cartilage in rat knee joints by CCN2 ( connective tissue growth factor) [J]. J Bone Miner Res, 2004, 19(8)∶1308-1319 |
| [17] | Kuiper EJ, Roestenberg P, Ehlken C, et al. Angiogenesis is not impaired in connective tissue growth factor (CTGF) knock-out mice [J]. J Histochem Cytochem, 2007, 55(11)∶1139-1147 |
| [18] | Kuiper EJ, Roestenberg P, Ehlken C, et al. Angiogenesis is not impaired in connective tissue growth factor (CTGF) knock-out mice [J]. J Histochem Cytochem, 2007, 55(11)∶1139-1147 |
| [19] | Jun JI, Lau LF. Taking aim at the extracellular matrix: CCN proteins as emerging therapeutic targets [J]. Nat Rev Drug Discov, 2011, 10(12)∶945-963 |
