|
|
- 2015
纯镁微弧氧化-HF-硅溶胶复合处理涂层细胞相容性研究
|
Abstract:
摘要 目的:对纯镁微弧氧化经HF-硅溶胶复合处理涂层进行成骨细胞生物相容性检测,评价纯镁表面微弧氧化经不同复合处理涂层后的细胞相容性。方法:实验分为3组,纯镁微弧氧化组为对照组(A组)、纯镁微弧氧化-硅溶胶复合处理组(B组)、纯镁微弧氧化-HF-硅溶胶复合处理组(C组)。将MC3T3-E1系成骨细胞接种在3组材料表面,分别通过扫描电镜、激光共聚焦、CCK-8、ALP对成骨细胞的生长、黏附、增殖以及活性进行检测。结果:扫描电镜和激光共聚焦显示B组和C组表面细胞的生长和黏附均优于A组,C组优于B组。CCK-8和ALP的检测结果显示3组材料表面细胞的增殖、活性为C组＞B组＞A组,3组的比较差异均具有统计学意义。结论:3组材料均具有良好的生物相容性,而纯镁微弧氧化-HF-硅溶胶复合处理后的生物相容性最佳
| [1] | Lin X, Tan L, Zhang Q, et al. The in vitro degradation process and bio-compatibility of a ZK60 magnesium alloy with forsterite-containing micro-arc oxidation coating [J]. Act a Biomaterialia, 2012 |
| [2] | Johnson I, Perchy D, Liu H. In vitro evaluation of the surface effects on magnesium-yttrium alloy degradation and mesenchymal stem cell adhesion [J]. J Biomed Mater Res Part A, 2011, 176(20)∶1778-1784 |
| [3] | Sun J, Li J, Liu X, et al. Proliferation and gene expression of osteoblasts cultured in DMEM containing the ionic products of dicalcium silicate coating [J]. Biomed Pharmacother, 2009, 63(9)∶650-657 |
| [4] | 杨柯,谭丽丽,任伊宾,等.AZ31镁合金的生物降解行为研究[J].中国材料进展,2009,28(2)∶26-30 |
| [5] | Witte F,Fischer J,Nellesen J,et al. In vivo corrosion and corrosion protection of Mg alloy LAE442 [J]. Acta Biomaterialia,2010,6(5)∶1792-1799 |
| [6] | Ripamonti U, Roden L C, Renton LF. Osteoinductive hydroxyapatite-coated titanium implants [J]. Biomaterials,2012,33(15)∶3813-3823 |
| [7] | 王亚明,王福会,雷廷权,等.镁合金生物陶瓷涂层在模拟体液中的腐蚀性能[J].热处理技术与装备,2007,28(6)∶6-9 |
| [8] | Xie Y, Zhai W, Chen L, et al. Preparation and in vitro evaluation of plasma-sprayed Mg2SiO4coating on titanium alloy [J]. Act a Biomater, 2009, 5(6)∶2331-2337 |
| [9] | Chiu KY, Wong MH, Cheng FT, et al. Characterization and corrosion studies of fiuoride conversion coating on degradable Mg implants [J]. Surf Coat Tech, 2007, 202(3)∶590-598 |
| [10] | 王树峰.AZ31镁合金修复兔下颌骨缺损[J].口腔医学研究,2013,29(1)∶33 |
| [11] | 刘鹤,陈春雨,何畔,等.镁材料的特性及医学研究进展[J].口腔医学研究,2013,29(9)∶874-875 |
| [12] | 李慕勤,刘江,蔡丁森,等.纯镁超声微弧氧化HF-Na<sub>2</sub>SiO<sub>3</sub>复合处理对生物涂层的影响[J].中国表面工程,2014,27(3)∶50-56 |
| [13] | Jugdaohsingh R. Silicon and bone health [J]. J Nutr Health Aging, 2007, 11(2)∶99-110 |
| [14] | Ni S, Chou L, Chang J. Preparation and characterization of forsterite (Mg2SiO4) bioceramics [J]. Ceram Inter, 2007, 33∶83-88 |
| [15] | Cooper LF,Zhou Y,Takebe J,et al. Fluoride modification effects on osteoblast behavior and bone formation at TiO2 grit-blasted c.p. titanium endosseous implants [J]. Biomaterials,2006,27(6)∶926-962 |
