|
|
- 2018
新疆博州地区维吾尔族、汉族儿童乳酸杆菌的分布及其与龋病的相关性研究
|
Abstract:
摘要 目的:研究新疆博州地区汉族、维吾尔族儿童口腔内乳酸杆菌的分布及其与龋病的相关性。方法:选用分层整群随机抽样方法抽取722名3~5岁健康儿童作为研究对象,分为3组:重度低龄儿童龋儿童281名,低龄儿童龋儿童283名,无龋儿童209名。采集牙菌斑样本,用Rogosa和MRS培养基选择性培养分离乳酸杆菌,经革兰染色、生化实验及聚合酶链式反应等方法进一步鉴定。Pearson χ2检验用于分类资料的统计分析。结果:总体样本中,乳酸杆菌检出率为63.2%;汉族儿童乳酸杆菌检出率69.5%高于维吾尔族58.2%(P=0.001);重度低龄儿童龋组乳酸杆菌检出率75.4%高于低龄儿童龋组66.8%和无龋40.4%(P=0.000);男性儿童乳酸杆菌检出率62.6%,女性63.8%,性别分布差异无统计学意义(P=0.794);3岁儿童乳酸杆菌检出率57.5%,4岁儿童65.2%与5岁儿童65.9%,差异无统计学意义(P=0.119)。结论:口腔内乳酸杆菌的分布存在民族差异;重度低龄儿童龋组、低龄儿童龋组和无龋组的乳酸杆菌分布有显著性差异;乳酸杆菌可能是龋病发生发展中的危险因素
| [1] | 侯鑫山,刘媛,张婉婷,等.新疆喀什、伊宁两地区维汉民族低龄儿龋流行病学调查分析[J].现代口腔医学杂志,2015,29(6)∶354-357 |
| [2] | Caufield PW, Schn CN, Saraithong P, et al. Oral lactobacilli and dental caries: a model for niche adaptation in humans [J]. J Dent Res,2015,94(9 Suppl)∶110S-118S |
| [3] | 仵楠,刘芳,赵今.维吾尔族、汉族婴幼儿口腔白色念珠菌的分布与龋病相关性研究[J].口腔医学研究,2013,29(4)∶338-341 |
| [4] | Yang R, Argimon S, Li Y, et al. Determining the genetic diversity of lactobacilli from the oral cavity [J]. J Microbiol Methods,2010,82(2)∶163-169 |
| [5] | Klinke T, Urban M, Lück C, et al. Changes in Candida spp. mutans streptococci and lactobacilli following treatment of early childhood caries: a 1-year follow-up [J]. Caries Res, 2013, 48(1)∶24-31 |
| [6] | Yao SG, Fine JB. Probiotics for bacterial disease treatment in the oral environment [J]. Compend Contin Educ Dent,2014,35(9)∶658-663 |
| [7] | Policy on Early Childhood Caries (ECC): Classifications, Consequences, and Preventive Strategies [J]. Pediatr Dent, 2011, 30(7 Suppl)∶31-33 |
| [8] | Tang RS, Huang MC, Huang ST, et al. Relationship between dental caries status and anemia in children with severe early childhood caries [J].Kaohsiung J Med Sci,2013,29(6)∶330-336 |
| [9] | Dubernet S, Desmasures N, Guéguen M, et al. A PCR-based method for identification of lactobacilli at the genus level [J]. FEMS Microbiol Lett, 2002, 214(2)∶271-275 |
| [10] | 冯岩,严恒,薛凯佳,等.高龋无龋儿童乳牙牙菌斑的微生物差异[J].口腔医学研究,2016,32(1)∶55-58 |
| [11] | Ramamurthy PH, Swamy HS, Bennete F, et al. Relationship between severe-early childhood caries, salivary mutans streptococci, and lactobacilli in preschool children of low socioeconomic status in Bengaluru city [J]. Int J Clin Pediatr Dent, 2014, 32(1)∶44 |
| [12] | 黄雅静,黄锐,钱英子,等.儿童口腔内变形链球菌、乳酸杆菌的检出情况分析[J].微生物学杂志,2012,32(2)∶106-109 |
| [13] | Chen L, Mao T, Du M, et al. Caries status and quantification of four bacteria in saliva of Chinese preschool children: A cross-sectional study [J].J Dent Sci,2014,9(3)∶283-288 |
| [14] | Sun Z, Harris HM, McCann A, et al. Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera [J]. Nat Commun,2015, 6∶8322 |
| [15] | Wasfi R, Abd El-Rahman OA, Zafer MM, et al. Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans [J]. J Cell Mol Med, 2018, 22(3)∶1972-1983 |
| [16] | Lif HP, hman C, Rnnlund A, et al. Maturation of oral microbiota in children with or without dental caries [J]. PLoS One,2015,10(5)∶e0128534 |
| [17] | 胡超.唾液中变形链球菌、乳酸杆菌与乳牙龋的相关性研究[D].中南大学,2012 |
