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- 2015
血竭素高氯酸盐对舌鳞癌细胞Tca-8113增殖及凋亡的影响
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Abstract:
摘要 目的:研究血竭素高氯酸盐(Dracorhodin perchlorate,DP)对舌鳞癌细胞Tca-8113增殖和凋亡的影响并初步探讨其相关机制。方法:以舌鳞癌细胞Tca-8113为研究对象,采用CCK-8、流式细胞术分析及Western Blot等方法检测不同浓度DP对Tca-8113细胞的增殖、凋亡及核因子E2-相关因子2(nuclear factor E2-related factor 2,Nrf2)、血红素氧合酶 1(heme oxygenase 1,HO-1)蛋白表达的变化。采用SPSS17.0统计软件对实验结果进行分析。结果:CCK-8检测表明,与空白对照组相比,DP可以显著抑制细胞增殖,且对细胞的增殖抑制作用有时间-剂量依赖性(P<0.05)。流式细胞术及Western Blot检测结果显示DP可以促进细胞凋亡(P<0.05),并提高Nrf2、HO-1在Tca-8113细胞中的表达。结论:DP能抑制舌鳞癌细胞Tca-8113增殖并诱导其发生凋亡,其作用可能与Nrf2/HO-1信号通路激活有关;可能成为抗口腔癌药物
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