|
|
- 2017
IL-24可能通过CXCL12/CXCR4信号轴抑制口腔鳞癌细胞迁移及侵袭能力
|
Abstract:
摘要 目的:探讨慢病毒介导IL-24基因转染口腔鳞癌细胞对细胞迁移和侵袭能力的影响及对CXCL12/CXCR4信号轴的影响。方法:培养OSCC细胞株,分为联合处理组、IL-24转染组、抑制剂组和空白对照组。转染后培养48 h,ELISA法检测各组细胞液中IL-24表达,检测不同处理组细胞细胞凋亡、迁移和侵袭能力,实时荧光定量PCR和Western blot法检测细胞中IL-24、CXCL12和CXCR4基因和蛋白表达。结果:联合处理组和IL-24转染组细胞上清液中IL-24水平均显著高于抑制剂组和空白对照组(P<0.05);联合处理组迁移数和侵袭数均低于IL-24转染组、抑制剂组和空白对照组,且IL-24转染组和抑制剂组均低于空白对照组(P<0.05);联合处理组和IL-24转染组细胞中IL-24 基因和蛋白表达量均高于抑制剂组和空白对照组(P<0.05),联合处理组和IL-24转染组细胞中CXCL12和CXCR4 基因和蛋白表达量均低于抑制剂组和空白对照组(P<0.05)。结论:上调IL-24可有效抑制OSCC细胞迁移及侵袭,可能与特异性抑制CXCL12/CXCR4信号通路有关
| [1] | 黄子轩,李红,代龙光,等.IL-24抑癌基因联合大蒜素对肺癌细胞的杀伤作用[J].吉林医药学院学报,2016,37(1)∶15-19 |
| [2] | Panneerselvam J, Shanker M, Jin J, et al. Phosphorylation of interleukin (IL)-24 is required for mediating its anti-cancer activity [J]. Oncotarget, 2015, 6(18)∶16271-16286 |
| [3] | 付丛,张行炜.长链非编码RNA AFAP1-AS1在口腔鳞状细胞癌中的表达及临床意义[J].口腔医学研究, 2016, 32(9)∶983-986 |
| [4] | Whitaker EL, Filippov VA, Duerksen-Hughes PJ. Interleukin 24: mechanisms and therapeutic potential of an anti-cancer gene [J]. Cytokine Growth Factor Rev, 2012, 23(6)∶323-331 |
| [5] | Jiang G, Zhang L, Xin Y, et al. Conditionally replicating adenoviruses carrying mda-7/IL-24 for cancer therapy [J]. Acta Oncol, 2012, 51(3)∶285-292 |
| [6] | Xu Q, Wang Z, Chen X, et al. Stromal-derived factor-1α/CXCL12-CXCR4 chemotactic pathway promotes perineural invasion in pancreatic cancer [J]. Oncotarget, 2015, 6(7)∶4717-4732 |
| [7] | Izumi D, Ishimoto T, Miyake K, et al. CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer [J]. Int J Cancer, 2016, 138(5)∶1207-1219 |
| [8] | 杨德钊,王丹丹,孟祥,等.病毒介导lL-24 基因对口腔鳞癌耐药细胞株凋亡的影响[J].口腔颌面外科杂志,2015,25(4)∶246-252 |
| [9] | Gualtero DF, Suarez Castillo A. Biomarkers in saliva for the detection of oral squamous cell carcinoma and their potential use for early diagnosis: a systematic review [J]. Acta Odontol Scand, 2016, 74(3)∶170-177 |
| [10] | Dash R, Bhutia SK, Azab B, et al. mda-7/IL-24: a unique member of the IL-10 gene family promoting cancer-targeted toxicity [J]. Cytokine Growth Factor Rev, 2010, 21(5)∶381-391 |
| [11] | 杨俊波,黄晓洁.白细胞介素-24通过CXC趋化因子配体12/CXC趋化因子受体4信号轴抑制肿瘤细胞转移[J].中华实验外科杂志,2015,32(10)∶2465-2468 |
