|
|
- 2015
甘肃东乡及裕固族牙周炎口腔微生物群落结构研究
|
Abstract:
摘要 目的:研究甘肃东乡族和裕固族成年人牙周炎唾液微生物群落结构。方法:构建16S rRNA克隆文库对测序结果进行分析,DP(东乡族样本),YP(裕固族样本)。结果:发现链球菌属存在于所有样本中;伴放线放线杆菌属(DP:0.00%, YP:3.30%)、奈瑟菌属(DP:10.40%, YP:3.60%)及韦永球菌属(DP:0.40%, YP:3.85%)在两组样本中的分布具有明显的差异(P＜0.05)。结论:甘肃东乡及裕固族牙周炎口腔微生物群落结构存在一定差异,其中,链球菌属、普氏菌属、梭杆菌属及嗜血杆菌属在牙周炎患者口腔中的作用值得进一步研究
| [1] | 李志强.中国东乡族、保安族、裕固族口腔疾病流行病学研究[D].兰州大学博士论文,2009 |
| [2] | Koyanagi T, Sakamoto M, Takeuchi Y, et al. Analysis of microbiota associated with peri-implantitis using 16S rRNA gene clone library [J]. Journal of oral microbiology, 2010, 2 |
| [3] | Xin BC, Luo AH, Qin J, et al. Microbial diversity in the oral cavity of healthy Chinese Han children [J]. Oral diseases, 2013, 19(4)∶401-405 |
| [4] | Zhang C, Hou BX, Zhao HY, et al. Microbial diversity in failed endodontic root-filled teeth [J]. Chin Med J (Engl), 2012, 125(6)∶1163-8 |
| [5] | Mason MR, Nagaraja HN, Camerlengo T, et al. Deep sequencing identifies ethnicity -specific bacterial signatures in the oral microbiome [J]. PloS one, 2013, 8(10)∶e77287 |
| [6] | Griffen A L, Beall CJ, Campbell JH, et al. Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing [J]. The ISME journal, 2011, 6(6)∶ 1176-1185 |
| [7] | Pizzo G, Guiglia R, Russo L L, et al. Dentistry and internal medicine: from the focal infection theory to the periodontal medicine concept [J]. European journal of internal medicine, 2010, 21(6)∶496-502 |
| [8] | Blaser MJ, Dominguez-Bello MG, Contreras M, et al. Distinct cutaneous bacterial assemblages in a sampling of South American Amerindians and US residents [J]. The ISME journal, 2012, 7(1)∶85-95 |
| [9] | Wang CY, Wang HC, Li JM, et al. Invasive infections of Aggregatibacter (Actinobacillus) actinomycetemcomitans [J]. Journal of Microbiology, Immunology and Infection, 2010, 43(6)∶491-497 |
| [10] | 齐小秋.第三次全国口腔健康流行病学调查报告[M].北京: 人民卫生出版社,2008∶6-20 |
| [11] | 曹莹,吕亚林,贾一新,等.心脏移植患者3种牙周可疑致病菌的检测[J].口腔医学研究,2014,6∶012 |
| [12] | Zhao L, Wang G, Siegel P, et al. Quantitative genetic background of the host influences gut microbiomes in chickens [J]. Scientific reports, 2013, 3 |
| [13] | 郝苏民.甘青特有民族文化形态研究[M].北京:民族出版社,1999 |
| [14] | 余世谦.中国饮食文化的民族传统[J].复旦学报(社会科学版),2002,5∶18-123,131 |
| [15] | Bik EM, Long CD, Armitage GC, et al. Bacterial diversity in the oral cavity of 10 healthy individuals [J]. The ISME journal, 2010, 4(8)∶962-974 |
| [16] | Belstrm D, Fiehn NE, Nielsen CH, et al. Differences in bacterial saliva profile between periodontitis patients and a control cohort [J]. Journal of clinical periodontology, 2014, 41(2)∶104-112 |
| [17] | Giannobile WV, McDevitt JT, Niedbala RS, et al. Translational and clinical applications of salivary diagnostics [J]. Advances in dental research, 2011, 23(4)∶375-380 |
| [18] | Jiang W, Zhang J, Chen H. Pyrosequencing analysis of oral microbiota in children with severe early childhood dental caries [J]. Current microbiology, 2013, 67(5)∶537-542 |
