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- 2015
不同加载频率拉应力对髁突软骨细胞增殖分化的影响
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Abstract:
摘要 目的:探讨不同加载频率的拉应力对髁突软骨细胞增殖、分化的影响。方法:体外分离2周龄新西兰兔髁突软骨细胞,对细胞施加2 h/d,为期3 d的不同频率(0 Hz、0.1 Hz、0.5 Hz、1 Hz)的拉应力。采用CCK8检测细胞增殖水平,实时荧光定量RT-PCR检测Sox9、Runx2、VEGF的表达。结果:细胞增殖水平在静止性(0 Hz)组显著高于周期性加力(0.1~1 Hz)组和对照组;Sox9、Runx2、VEGF表达在0.5 Hz组和1 Hz组显著高于静止性(0 Hz)组和对照组。结论:不同加载频率拉应力对髁突软骨细胞增殖分化有差异性影响
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