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- 2018
Mongersen在牙周炎症微环境中抗炎作用的体外研究
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Abstract:
摘要 目的: 探讨Smad7反义寡核苷酸mongersen的体外抗炎作用。方法: 使用LipofectamineTM，2000载mongersen转染小鼠巨噬细胞RAW264.7,之后将RAW264.7与牙周炎的主要致病菌牙龈卟啉单胞菌的脂多糖(Porphyromonas gingivlis lipopolysaccharide,Pg-LPS)共培养,以模拟牙周炎症微环境,共培养12 h后,采用流式细胞术、Western blot、qRT-PCR分别检测转染效率、Smad7蛋白的表达情况及巨噬细胞相关细胞因子的表达。结果: 流式细胞术检测转染效率可达76.57%;Western blot结果显示mongersen可明显抑制Smad7蛋白的表达;qRT-PCR结果显示加入mongersen后肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)等促炎因子基因表达明显降低。结论: mongersen可通过抑制Smad7蛋白的表达发挥抗炎作用
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