|
|
- 2018
一种新型牙骨质细胞系的矿化特性研究
|
Abstract:
摘要 目的: 探究一种新型的牙骨质样细胞系IDG-CM6的矿化特性。方法: 体内实验采用3月龄C57BL/6雄性小鼠下颌第一磨牙近中根的细胞牙骨质和牙槽骨扫描电镜(scanning electron microscope,SEM)及共聚焦显微拉曼光谱为参考;体外培养以骨细胞系IDG-SW3细胞为对照,对矿化3、7、14、21和28 d的IDG-CM6细胞及IDG-SW3细胞进行茜素红染色、SEM及共聚焦显微拉曼光谱扫描,研究其矿化特性。结果: 同一时间节点的茜素红染色结果显示,两种细胞均在培养中出现明显矿化,IDG-CM6细胞钙离子沉积更加明显。SEM及能谱仪(energy dispersive spectrometer,EDS)分析显示,两种细胞的Ca/P比差异无统计学意义,与体内实验结果一致。拉曼分析结果显示相同矿化节点,IDG-CM6细胞的PO43-/CH2(矿化成分/有机基质)比值显著低于IDG-SW3细胞,这与体内细胞牙骨质的PO43-/CH2比值(1.76)明显低于牙槽骨(4.76)一致。结论: 新型牙骨质细胞系IDG-CM6细胞可能是研究牙骨质矿化特征及生物学特性的理想体外模型
| [1] | Zhao N, Foster BL, Bonewald LF. The cementocyte- an osteocyte relative?[J]. J Dent Res, 2016, 95(7)∶734-741 |
| [2] | Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing and regeneration [J]. Crit Rev Oral Biol Med, 2002, 13(6)∶474-484 |
| [3] | Dallas SL, Prideaux M, Bonewald LF. The osteocyte: an endocrine celland more [J]. Endocr Rev, 2013, 34(5)∶658-690 |
| [4] | Wang C, Wang Y, Huffman NT, et al. Confocal laser Raman microspectroscopy of biomineralization foci in UMR 106 osteoblastic cultures reveals temporally synchronized protein changes preceding and accompanying mineral crystal deposition [J]. J Biol Chem, 2009, 284(11)∶7100-7113 |
| [5] | Bosshardt DD, Selvig KA. Dental cementum: the dynamic tissue covering of the root [J]. Periodontol 2000,1997,13∶41-75 |
| [6] | 廖乃顺,李钻芳,林如辉,等.比较3种形态学方法观察成骨细胞矿化结节的应用价值[J].中国组织工程研究,2014,18(33)∶5266-5270 |
| [7] | 柳康,王越,彭慧敏,等.大鼠下颌下腺细胞培养及其生物学特性研究[J].口腔医学研究,2016,32(8)∶816-818 |
| [8] | Wang W, Zhao J, Short M, et al. Real-time <em>in vivo</em> cancer diagnosis using raman spectroscopy [J]. J Biophotonics, 2015, 8(7)∶527-545 |
| [9] | Puppels GJ, Colier W, Olminkhof JHF, et al. Description and performance of a highly sensitive confocal Raman microspectrometer [J]. J Raman Spectrosc, 2017, 22(4)∶217-225 |
| [10] | Yamamoto T, Hasegawa T, Yamamoto T, et al. Histology of human cementum: Its structure, function, and development [J]. Jpn Dent Sci Rev, 2016, 52(3)∶63-74 |
| [11] | Zhao N, Nociti FH, Duan P, et al. Isolation and functional analysis of an immortalized murine cementocyte cell line, IDG-CM6 [J]. J Bone Miner Res, 2016, 31(2)∶430-442 |
| [12] | Bonewald LF. The amazing osteocyte [J]. J Bone Miner Res, 2011, 26(2)∶229-238 |
| [13] | Lanza D, Perna AF, Oliva A, et al. Impact of the uremic milieu on the osteogenic potential of mesenchymal stem cells [J]. PLoS One, 2015, 10(1)∶e0116468 |
| [14] | Mcgregor HC, Short MA, Mcwilliams A, et al. Real-time endoscopic Raman spectroscopy for <em>in vivo</em> early lung cancer detection [J]. J Biophotonics, 2017, 10(1)∶98-110 |
| [15] | Brauchle E, Thude S, Brucker SY, et al. Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy [J]. Sci Rep, 2014, 4∶4698 |
| [16] | Imai Y, Hasegawa T, Takeda D, et al. The osteogenic activity of human mandibular fracture haematoma-derived cells is stimulated by low-intensity pulsed ultrasound <em>in vitro</em> [J]. Int J Oral Maxillofac Surg, 2014, 43(3)∶367-372 |
| [17] | 章晓霜,唐键,屈菊兰.不同强度运动对去卵巢大鼠股骨下端松质骨影响的扫描电镜观察[J].中国运动医学杂志,2002,21(1)∶48-50 |
