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- 2018
槲皮素对人口腔上皮细胞再生的生物学效应研究
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Abstract:
摘要 目的: 研究槲皮素对人口腔角质细胞(human oral keratinocytes,HOKs)再生的生物学效应。方法: 通过细胞计数试剂盒-8(cell counting kit-8,CCK-8)及迁移实验验证槲皮素对人牙龈角质细胞的增殖与迁移的药理作用。通过牙龈卟啉单胞菌脂多糖作为炎症刺激因子构建体外炎症模型,实时荧光定量聚合酶链反应(real time quantitative polymerase chain reaction,RT-qPCR)法检测转化生长因子(transforming growth factor,TGF)-β1和TGF-β3的表达及炎症因子白细胞介素(interleukin,IL)-1β和肿瘤坏死因子(tumor necrosis factor,TNF)-α的基因表达验证槲皮素的抗炎效果。酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测TGF-β1和TGF-β3的分泌水平。结果: 20 μmol/L槲皮素促进HOKs的增殖与迁移,并提高TGF-β3的mRNA水平与蛋白质表达,而50 μmol/L槲皮素对TGF-β1的表达有促进作用。20、50 μmol/L的槲皮素都有明显的抗炎作用。结论: 20 μmol/L槲皮素在体外实验中能够促进创面愈合并且促进HOKs再生
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