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- 2018
牙龈卟啉单胞菌对血管平滑肌细胞钙化作用的研究
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Abstract:
摘要 目的:探讨牙龈卟啉单胞菌(Porphyromonasgingivalis, P. gingivalis)对小鼠血管平滑肌细胞(vascular smooth muscle cell, VSMC)钙化的影响。方法:选取C57BL/6小鼠,取主动脉体外培养VSMC,通过细胞免疫荧光染色,用α-smooth muscle actin(α-SMA)特异性抗体对VSMC鉴定。热灭活P. gingivalis以不同的感染倍数(multiplicity of infection,MOI)干预VSMC,分别在12、24、48、72 h检查VSMC的细胞活性,在21 天时检测VSMC钙化表现及钙含量。并且将P. gingivalis与小鼠主动脉共培养,观察P. gingivalis对小鼠主动脉的钙化作用。结果:特异性抗体α-SMA在细胞中荧光染色阳性,证明体外培养为VSMC。P. gingivalis(MOI:10,100)刺激VSMC在24和48 h,细胞活性明显增强,在72 h,所有感染倍数下(MOI:1,10,100 )细胞活性较对照组均明显增强(P<0.05)。培养至21 d时,P. gingivalis诱导VSMC出现明显钙化,钙含量与MOI成正相关(P<0.05)。P. gingivalis与小鼠主动脉培养14 d,见主动脉壁出现明显钙化沉积。结论:P. gingivalis可诱导VSMC细胞活性增强及钙化,并且可直接引起主动脉壁钙化沉积
| [1] | Neil J. Paloian, Cecilia M. Giachelli. A current understanding of vascular calcification in CKD [J]. Am J Physiol Renal Physiol, 2014, 307(8)∶F891-F900 |
| [2] | Hu L, Boesten LS, May P, 等.巨噬细胞低密度脂蛋白受体相关蛋白缺乏促进载脂蛋白E/低密度脂蛋白受体双基因敲除小鼠动脉粥样硬化的形成[J]. 中国动脉硬化杂志,2007,4∶292 |
| [3] | Chie H, Jason V, Ning H, et al. Porphyromonasgingivalis accelerates inflammatory atherosclerosis in the innominate artery of ApoE deficient mice[J]. Atherosclerosis,2011,215(1)∶52-59 |
| [4] | 李爽,易根云,李志强,等.牙周炎与冠心病之间联系机制等研究进展 [J].口腔医学研究,2017,33(4)∶455-458 |
| [5] | 吴贇,陈凌,魏斌,等.龈下及动脉粥样硬化斑块中牙周致病菌的同源性分析 [J].口腔医学研究,2013,29(6)∶543-546 |
| [6] | Jyoti CG, Varsha S, Debaleena B, et al. Linkages between oral commensal bacteria and atherosclerotic plaques in coronary artery disease patients [J]. NPJ Biofilms Microbiomes,2016,2∶7-14 |
| [7] | Jane A. Leopold. Vascular Calcification: Mechanisms of Vascular Smooth Muscle Cell Calcification[J]. Trends Cardiovasc Med,2015,25(4)∶267-274 |
| [8] | Dzau VJ, Braun-Dullaeus RC, Sedding DG. Vascular proliferation and atherosclerosis: new perspectives and therapeutic strategies[J]. Nat Med,2002,8(11)∶1249-1256 |
| [9] | Wei L, Yue Z, CM Y, et al. Current understanding of coronary artery calcification [J]. J Geiatr Cardiol, 2015,12(6)∶668-675 |
| [10] | Bessueille L, Magne D. Inflammation: a culprit for vascular calcification in atherosclerosis and diabetes [J].Cell Mol Life Sci, 2015, 72(13)∶2475-2489 |
| [11] | Jaideep M, Little M, John F. Prevelance of periodontopathogenic bacteria in subgingival biofilm and atherosclerotic plaques of patients undergoing coronary revascularization surgery [J]. J Indian Soc Periodontol[J]. 2013,17(6)∶719-724 |
| [12] | Chie H, Jason V, Ning H, et al. Porphyromonasgingivalis Accelerates Inflammatory Atherosclerosis in the Innominate Artery of ApoE Deficient Mice [J]. Atherosclerosis, 2011, 215(1)∶52-59 |
| [13] | Sasanka S, Irina M, Mercedes F, et al. Polymicrobial Oral Infection with Four Periodontal Bacteria Orchestrates a Distinct Inflammatory Response and Atherosclerosis in Apo Enull Mice [J]. Plos One,2015,10(11)∶e0143291 |
| [14] | Panh L, Lairez O, Ruidavets JB, et al. Vascular Damage and Kidney Transplant Outcomes: An Unfriendly and Harmful Link [J]. Am J Med Sci, 2017, 54(1)∶7-16 |
| [15] | Tang L, Zhou XD, Wang Q, et al. TNF receptor-associated factor 6 suppression inhibits inflammatory response to Porphyromonasgingivialis in human periodontal ligament cells[J]. Quintessence Int, 2011, 42(9)∶787-796 |
| [16] | Kapustin AN, Chatrou ML, Drozdov I, et al. Vascular smooth muscle cell calcification is mediated by regulated exosome secretion[J]. Circ Res,2015,116(8)∶1312-1323 |
| [17] | Yong S, Byon CH, Yang Y, et al. Dietary potassium regulates vascular calcification and arterial stiffness [J]. JCI Insight, 2017, 2(19)∶pii94920 |
