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- 2018
口腔鳞状细胞癌患者外周血淋巴细胞免疫分型及临床意义
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Abstract:
摘要 目的:研究口腔鳞状细胞癌(Oral squamous cell carcinoma,OSCC)患者外周血中不同淋巴细胞免疫表型的分布特征,探讨其与癌变相关危险因素及肿瘤生物学行为之间的关系。方法:选取49例口腔鳞状细胞癌患者和15例正常成人的外周血,应用流式细胞术检测外周血中T、B淋巴细胞及其各免疫表型的分布特征,并对其与槟榔咀嚼、吸烟、饮酒等癌变危险因素及肿瘤TNM分期和淋巴结转移相关性进行分析。结果:OSCC患者外周血中CD8+T细胞、效应记忆T细胞(CD4+Teff、 CD8+Teff)、CD4+PD1+T、CD8+PD1+T、CD8+PD1+Teff、CD8+Tim3+T、CD8+Tim3+Teff的表达均显著高于正常对照组,而CD4+初始T细胞(Tn)、B细胞则显著降低,且CD4+Tn/CD4+Teff、CD8+Tn/CD8+Teff比值均明显下降(P<0.05);OSCC患者外周血中CD8+T细胞、CD4+Teff、CD8+Teff细胞的增高与淋巴结转移及肿瘤分期显著相关,且伴淋巴结转移或中晚期患者外周血中CD4+Tn细胞及CD4/CD8、CD4+Tn/Teff的比例均显著降低;OSCC患者外周血中CD4+Teff比例的降低和CD4+Tn细胞比例的升高与槟榔咀嚼显著相关(P<0.05),而饮酒和吸烟与T、B细胞各免疫亚型的改变无显著相关。结论:本研究发现OSCC患者外周血中淋巴细胞各免疫表型发生了改变,且这种改变与槟榔咀嚼及肿瘤进展显著相关
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