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- 2018
p-Akt对牙龈卟啉单胞菌诱导的人胚胎滋养层细胞凋亡的影响
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Abstract:
摘要 目的:探讨牙龈卟啉单胞菌对胚胎滋养层细胞的影响及p-Akt在此过程中的作用。方法:牙龈卟啉单胞菌ATCC33277(Porphyromonas gingivalis)以感染复数(MOI)为200∶1与人胚胎滋养层细胞(HTR-8)共培养,以单纯培养人胚胎滋养层细胞为对照组;采用siRNA失活PI3K;选择倒置显微镜和共聚焦显微镜记录人胚胎滋养层细胞的形态变化;采用流式细胞术检测感染后人胚胎滋养层细胞凋亡的情况;用Real Time-PCR检测PI3K siRNA敲低的效果。结果:牙龈卟啉单胞菌感染人胚胎滋养层细胞12 h时,细胞的凋亡率与正常组相比无统计学意义;感染24 h时,细胞凋亡明显增多(P<0.01);感染48 h时,细胞凋亡与对照组相比更为明显(P<0.001)。siRNA预先处理HTR-8细胞再与牙龈卟啉单胞菌共培养48 h后,siRNA处理组的PI3K mRNA的相对量明显低于干扰组(P<0.01);同时,细胞凋亡率>45%,明显高于单纯感染牙龈卟啉单胞菌组(P<0.01),而干扰组的细胞凋亡率与单纯刺激组相比无统计学差异。结论:牙龈卟啉单胞菌能够诱导胚胎滋养层细胞发生凋亡。在此过程中,p-Akt被激活,并对凋亡有一定的抑制作用。提示,牙周炎促使早产低体重儿的娩出与牙龈卟啉单胞菌诱导胚胎滋养层细胞凋亡有关
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