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- 2018
抑制白色念珠菌用载氟康唑明胶纤维支架的制备及表征
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Abstract:
摘要 目的:制备载氟康唑明胶纤维支架,作为抑制白色念珠菌生长的新型制剂,探讨其理化性能及生物性能。方法:采用静电纺丝方法制备载氟康唑明胶纤维支架,在扫描电镜下观察其形貌表征;通过体外降解实验测试其稳定性;紫外分光光度检测氟康唑释放量;细胞黏附实验观察其黏附性;MTT检测生物安全性;体外培养白色念珠菌测试其抑制白色念珠菌性能。结果:载氟康唑明胶纤维支架具有纳米级纤维直径,呈交错的三维网络结构,5 d时体外降解量约为10%;细胞存活率高,部分细胞黏附在支架表面,部分伸入其中;氟康唑在6 h内释放较快,此后缓慢释放;支架材料对白色念珠菌具有抑制作用。结论:载氟康唑明胶纤维支架的稳定性、黏附性及生物安全性好,药物释放量大而持久,抑制白色念珠菌性能优异
[1] | Ohshima T, Kojima Y, Seneviratne CJ, et al. Therapeutic application of synbiotics, a fusion of probiotics and prebiotics, and biogenics as a new concept for oral candida infections: a mini review [J]. Front Microbiol, 2016, 7∶10 |
[2] | Pappas PG, Kauffman CA, Andes DR, et al. Clinical practice guideline for the management of candidiasis: 2016 update by the infectious diseases society of America [J]. Clin Infect Dis, 2016, 62(4)∶e1-e50 |
[3] | Berkow EL, Lockhart SR. Fluconazole resistance in Candida species: a current perspective [J]. Infect Drug Resist, 2017, 10∶237-245 |
[4] | Khanvilkar K, Donovan MD, Flanagan DR. Drug transfer through mucus [J]. Adv Drug Deliv Rev, 2001, 48(2-3)∶173-193 |
[5] | Zafar M, Najeeb S, Khurshid Z, et al. Potential of electrospun nanofibers for biomedical and dental applications [J]. Materials (Basel), 2016, 9(2). pii: E73 |
[6] | Yehia SA, El-Gazayerly ON, Basalious EB. Design and <i>in vitro</i>/<i>in vivo</i> evaluation of novel mucoadhesive buccal discs of an antifungal drug: relationship between swelling, erosion, and drug release [J]. AAPS Pharm SciTech, 2008, 9(4)∶1207-1217 |
[7] | 陈谦明.口腔黏膜病学[M].第4版.北京:人民卫生出版社,2012∶34-42 |
[8] | Akbar E, Syed NM. Synthesis of a novel structure for the oral delivery of insulin and the study of its effect on diabetic rat [J]. Life Sci, 2017, 186∶43-49 |
[9] | Tamás V, Tímea H, Attila B, et al. Polymer-free and polyvinylpirrolidone-based electrospun solid dosage forms for drug dissolution enhancement[J]. Eur J Pharm Sci, 2013, 49(4)∶595-602 |
[10] | Ainola M, Tomaszewski W, Ostrowska B, et al. A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair [J]. J Biomater Appl, 2016, 30(6)∶873-885 |
[11] | 张震,李莹,耿亚伟,等.纳米支架及合成技术在神经组织再生的应用[J].口腔医学研究,2017,33(4)∶459-462 |
[12] | Ranjbar-Mohammadi M, Zamani M, Prabhakaran M, et al. Electrospinning of PLGA/gum tragacanth nanofibers containing tetracycline hydrochloride for periodontal regeneration [J]. Mater Sci Eng C Mater Biol Appl, 2016, 58∶521-531 |
[13] | Pawar S, Pande V. Oleic acid coated gelatin nanoparticles impregnated gel for sustained delivery of zaltoprofen: formulation and textural characterization [J]. Adv Pharm Bull, 2015, 5(4)∶537-548 |
[14] | Huang CY, Hu KH, Wei ZH. Comparison of cell behavior on pva/pva-gelatin electrospun nanofibers with random and aligned configuration [J]. Sci Rep, 2016, 6∶37960 |
[15] | Ji W, Sun Y, Yang F, et al. Bioactive electrospun scaffolds delivering growth factors and genes for tissue engineering applications [J]. Pharm Res, 2011, 28(6)∶1259-1272 |